A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine

被引：57
作者：
Fonseca, Erica Lourenco [1 ]
Ramos, Nilceia da Veiga [1 ]
Nascimento Andrade, Bruno G. [1 ]
Morais, Lena L. C. S. [2 ]
Abanto Marin, Michel F. [1 ]
Vicente, Ana Carolina P. [1 ]
机构：
[1] Fiocruz MS, Inst Oswaldo Cruz, Lab Genet Mol Microrganismos, Ave Brasil 4365, Manguinho, RJ, Brazil
[2] Inst Evandro Chagas, Secao Meio Ambiente, Lab Microbiol Ambiental, Belem, Para, Brazil
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 87卷 / 04期
关键词：
Class A beta-lactamase; Klebsiella species; Molecular identification; bla(LEN); bla(SHV); bla(OKP); SUBSP QUASIPNEUMONIAE; IDENTIFICATION; INFECTION; DIVERSITY;
D O I：
10.1016/j.diagmicrobio.2017.01.005
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for bla(SHV), bia(LEN) and bla(OKP) and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：315 / 317
页数：3
相关论文
共 16 条
[1]   Draft Genome Sequence of the First Hypermucoviscous Klebsiella quasipneumoniae subsp. quasipneumoniae Isolate from a Bloodstream Infection [J].
Arena, Fabio ;
De Angelis, Lucia Henrici ;
Pieralli, Filippo ;
Di Pilato, Vincenzo ;
Giani, Tommaso ;
Torricelli, Francesca ;
D'Andrea, Marco Maria ;
Rossolini, Gian Maria .
GENOME ANNOUNCEMENTS, 2015, 3 (05)
[2]   An Investigation into Laboratory Misidentification of a Bloodstream Klebsiella variicola Infection [J].
Berry, Gregory J. ;
Loeffelholz, Michael J. ;
Williams-Bouyer, Natalie .
JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (08) :2793-2794
[3]   Liver Abscess Caused by Infection with Community-Acquired Klebsiella quasipneumoniae subsp quasipneumoniae [J].
Breurec, Sebastien ;
Melot, Benedicte ;
Hoen, Bruno ;
Passet, Virginie ;
Schepers, Kinda ;
Bastian, Sylvaine ;
Brisse, Sylvain .
EMERGING INFECTIOUS DISEASES, 2016, 22 (03) :529-531
[4]   Development of a rapid identification method for Klebsiella pneumoniae phylogenetic groups and analysis of 420 clinical isolates [J].
Brisse, S ;
van Himbergen, T ;
Kusters, K ;
Verhoef, J .
CLINICAL MICROBIOLOGY AND INFECTION, 2004, 10 (10) :942-945
[5]   Phylogenetic diversity of Klebsiella pneumoniae and Klebsiella oxytoca clinical isolates revealed by randomly amplified polymorphic DNA, gyrA and parC genes sequencing and automated ribotyping [J].
Brisse, S ;
Verhoef, J .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :915-924
[6]   Description of Klebsiella quasipneumoniae sp nov., isolated from human infections, with two subspecies, Klebsiella quasipneumoniae subsp quasipneumoniae subsp nov and Klebsiella quasipneumoniae subsp similipneumoniae subsp nov., and demonstration that Klebsiella singaporensis is a junior heterotypic synonym of Klebsiella variicola [J].
Brisse, Sylvain ;
Passet, Virginie ;
Grimont, Patrick A. D. .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2014, 64 :3146-3152
[7]   Genomic identification of nitrogen-fixing Klebsiella variicola, K. pneumoniae and K. quasipneumoniae [J].
Chen, Mingyue ;
Li, Yuanyuan ;
Li, Shuying ;
Tang, Lie ;
Zheng, Jingwu ;
An, Qianli .
JOURNAL OF BASIC MICROBIOLOGY, 2016, 56 (01) :78-84
[8]   Draft Genome Sequence of the First Hypermucoviscous Klebsiella variicola Clinical Isolate [J].
Garza-Ramos, Ulises ;
Silva-Sanchez, Jesus ;
Barrios, Humberto ;
Rodriguez-Medina, Nadia ;
Martinez-Barnetche, Jesus ;
Andrade, Veronica .
GENOME ANNOUNCEMENTS, 2015, 3 (02)
[9]   Diversity and evolution of the class a chromosomal beta-lactamase gene in Klebsiella pneumoniae [J].
Hæggman, S ;
Löfdahl, S ;
Paauw, A ;
Verhoef, J ;
Brisse, S .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (07) :2400-2408
[10]   Klebsiella variicola Is a Frequent Cause of Bloodstream Infection in the Stockholm Area, and Associated with Higher Mortality Compared to K. pneumoniae [J].
Maatallah, Makaoui ;
Vading, Malin ;
Kabir, Muhammad Humaun ;
Bakhrouf, Amina ;
Kalin, Mats ;
Naucler, Pontus ;
Brisse, Sylvain ;
Giske, Christian G. .
PLOS ONE, 2014, 9 (11)
12