Designed signaling aptamers that transduce molecular recognition to changes in fluorescence intensity

被引：239

作者：

Jhaveri, SD

Kirby, R

Conrad, R

Maglott, EJ

Bowser, M

Kennedy, RT

Glick, G

Ellington, AD ^{[1
]}

机构：

[1] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA

[2] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[3] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[4] Univ Florida, Dept Chem, Gainesville, FL 32611 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2000年 / 122卷 / 11期

关键词：

D O I：

10.1021/ja992393b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have engineered aptamers that contain fluorescent reporters and that signal the presence of cognate ligands in solution. Two different anti-adenosine "signaling aptamers", one made from RNA and one from DNA, can selectively signal the presence of adenosine in solution. Increases in fluorescence intensity reproducibly follow increases in adenosine concentration, and can be used for quantitation. The facile methods we have developed can potentially be used for generating a wide variety of signaling aptamers for use in sensor arrays.

引用

页码：2469 / 2473

页数：5

共 23 条

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