Simultaneous single-molecule discrimination of cysteine and homocysteine with a protein nanopore

被引：36
|
作者：
Lu, Yao [1 ]
Wu, Xue-Yuan [1 ]
Ying, Yi-Lun [1 ,2 ]
Long, Yi-Tao [1 ,2 ]
机构：
[1] East China Univ Sci, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China
[2] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 63期
基金：
中国国家自然科学基金;
关键词：
FLUORESCENCE DETECTION; AEROLYSIN NANOPORE; IDENTIFICATION; GLUTATHIONE;
D O I：
10.1039/c9cc04077c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Discrimination between cysteine and homocysteine at the single-molecule level is achieved within a K238Q mutant aerolysin nanopore, which provides a confined space for high spatial resolution to identify the amino acid difference with a 5 '-benzaldehyde poly(dA)(4) probe. Our strategy allows potential detection and characterization of various amino acids and their modifications, and provides a crucial step towards developing nanopore protein sequencing devices.
引用
收藏
页码：9311 / 9314
页数：4
相关论文
共 50 条
  • [21] Single-molecule resolution of macromolecules with nanopore devices
    Ren, Meili
    Liu, Daixin
    Qin, Fupeng
    Chen, Xun
    Ma, Wenhao
    Tian, Rong
    Weng, Ting
    Wang, Deqang
    Astruc, Didier
    Liang, Liyuan
    ADVANCES IN COLLOID AND INTERFACE SCIENCE, 2025, 338
  • [22] Single-Molecule Study on Interactions between Cyclic Nonribosomal Peptides and Protein Nanopore
    Zhou, Shuo
    Wang, Han
    Chen, Xiaohan
    Wang, Yunjiao
    Zhou, Daming
    Liang, Liyuan
    Wang, Liang
    Wang, Deqiang
    Guan, Xiyun
    ACS APPLIED BIO MATERIALS, 2020, 3 (01): : 554 - 560
  • [23] Single-molecule post-translational modification profiling with nanopore protein sequencing
    Ritmejeris, Justas
    Chen, Xiuqi
    Sande, Jasper W. V. D.
    Brinkerhoff, Henry
    Laszlo, Andrew H.
    Albada, Bauke
    Dekker, Cees
    BIOPHYSICAL JOURNAL, 2025, 124 (03) : 502A - 502A
  • [24] Multi-pass, single-molecule nanopore reading of long protein strands
    Motone, Keisuke
    Kontogiorgos-Heintz, Daphne
    Wee, Jasmine
    Kurihara, Kyoko
    Yang, Sangbeom
    Roote, Gwendolin
    Fox, Oren E.
    Fang, Yishu
    Queen, Melissa
    Tolhurst, Mattias
    Cardozo, Nicolas
    Jain, Miten
    Nivala, Jeff
    NATURE, 2024, : 662 - 669
  • [25] Single-Molecule Characterization of DNA-Protein Interactions Using Nanopore Biosensors
    Squires, A. H.
    Gilboa, T.
    Torfstein, C.
    Varongchayakul, N.
    Meller, A.
    SINGLE-MOLECULE ENZYMOLOGY: NANOMECHANICAL MANIPULATION AND HYBRID METHODS, 2017, 582 : 353 - 385
  • [26] Chemically functionalized conical PET nanopore for protein detection at the single-molecule level
    Zhang, Youwen
    Chen, Xiaohan
    Wang, Ceming
    Roozbahani, Golbarg M.
    Chang, Hsueh-Chia
    Guan, Xiyun
    BIOSENSORS & BIOELECTRONICS, 2020, 165
  • [27] A generalizable nanopore sensor for highly specific protein detection at single-molecule precision
    Ahmad, Mohammad
    Ha, Jeung-Hoi
    Mayse, Lauren A.
    Presti, Maria F.
    Wolfe, Aaron J.
    Moody, Kelsey J.
    Loh, Stewart N.
    Movileanu, Liviu
    NATURE COMMUNICATIONS, 2023, 14 (01)
  • [28] Nanopore approaches to single-molecule proteomics: From protein sequence to enzyme dynamics
    Maglia, Giovanni
    BIOPHYSICAL JOURNAL, 2024, 123 (03) : 8A - 8A
  • [29] A generalizable nanopore sensor for highly specific protein detection at single-molecule precision
    Mohammad Ahmad
    Jeung-Hoi Ha
    Lauren A. Mayse
    Maria F. Presti
    Aaron J. Wolfe
    Kelsey J. Moody
    Stewart N. Loh
    Liviu Movileanu
    Nature Communications, 14
  • [30] Multi-channel nanopore sensing platform for single-molecule protein fingerprinting
    Makhamreh, Amr K.
    Fallahi, Ali
    Zhang, Zhuoyu
    Wanunu, Meni
    BIOPHYSICAL JOURNAL, 2024, 123 (03) : 146A - 146A
12345