Simultaneous single-molecule discrimination of cysteine and homocysteine with a protein nanopore

被引：37

作者：

Lu, Yao ^{[1
]}

Wu, Xue-Yuan ^{[1
]}

Ying, Yi-Lun ^{[1
,2
]}

Long, Yi-Tao ^{[1
,2
]}

机构：

[1] East China Univ Sci, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

[2] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 63期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENCE DETECTION; AEROLYSIN NANOPORE; IDENTIFICATION; GLUTATHIONE;

D O I：

10.1039/c9cc04077c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Discrimination between cysteine and homocysteine at the single-molecule level is achieved within a K238Q mutant aerolysin nanopore, which provides a confined space for high spatial resolution to identify the amino acid difference with a 5 '-benzaldehyde poly(dA)(4) probe. Our strategy allows potential detection and characterization of various amino acids and their modifications, and provides a crucial step towards developing nanopore protein sequencing devices.

引用

页码：9311 / 9314

页数：4

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