Simultaneous single-molecule discrimination of cysteine and homocysteine with a protein nanopore

被引：36
|
作者：
Lu, Yao [1 ]
Wu, Xue-Yuan [1 ]
Ying, Yi-Lun [1 ,2 ]
Long, Yi-Tao [1 ,2 ]
机构：
[1] East China Univ Sci, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China
[2] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 63期
基金：
中国国家自然科学基金;
关键词：
FLUORESCENCE DETECTION; AEROLYSIN NANOPORE; IDENTIFICATION; GLUTATHIONE;
D O I：
10.1039/c9cc04077c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Discrimination between cysteine and homocysteine at the single-molecule level is achieved within a K238Q mutant aerolysin nanopore, which provides a confined space for high spatial resolution to identify the amino acid difference with a 5 '-benzaldehyde poly(dA)(4) probe. Our strategy allows potential detection and characterization of various amino acids and their modifications, and provides a crucial step towards developing nanopore protein sequencing devices.
引用
收藏
页码：9311 / 9314
页数：4
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