The effect of surface microtopography of poly(dimethylsiloxane) on protein adsorption, platelet and cell adhesion

被引:76
作者
Chen, Hong [1 ]
Song, Wei
Zhou, Feng
Wu, Zhongkui
Huang, He
Zhang, Junhu [2 ]
Lin, Quan [2 ]
Yang, Bai [2 ]
机构
[1] Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Sch Mat Sci & Engn, Wuhan 430070, Peoples R China
[2] Jilin Univ, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China
基金
芬兰科学院;
关键词
Surface topography; Protein adsorption; Platelet adhesion; Cell's morphology; Poly(dimethylsiloxane); TRANSFER RADICAL POLYMERIZATION; SOFT LITHOGRAPHY; NANOMETER-SCALE; FIBRONECTIN ADSORPTION; COLLOIDAL CRYSTALS; ADSORBED PROTEIN; ANTIBODY-BINDING; TOPOGRAPHY; FIBRINOGEN; PROLIFERATION;
D O I
10.1016/j.colsurfb.2009.02.018
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Chemical homogeneous poly(dimethylsiloxane) (PDMS) surface with dot-like protrusion pattern was used to investigate the individual effect of surface microtopography on protein adsorption and subsequent biological responses. Fibrinogen (Fg) and fibronectin (Fn) were chosen as model proteins due to their effect on platelet and cell adhesion, respectively. Fg labeled with (125)I and fluorescein isothiocyanate (FITC) was used to study its adsorption on flat and patterned surfaces. Patterned surface has a 46% increase in the adsorption of Fg when compared with flat surface. However, the surface area of the patterned surface was only 8% larger than that of the flat surface. Therefore, the increase in the surface area was not the only factor responsible for the increase in protein adsorption. Clear fluorescent pattern was visualized on patterned surface, indicating that adsorbed Fg regularly distributed and adsorbed most on the flanks and valleys of the protrusions. Such distribution and local enrichment of Fg presumably caused the specific location of platelets adhered from platelet-rich plasma (PRP) and flowing whole blood (FWB) on patterned surface. Furthermore, the different combination of surface topography and pre-adsorbed Fn could influence the adhesion of L929 cells. The flat surface with pre-adsorbed Fn was the optimum substrate while the virgin patterned surface was the poor substrate in terms of L929 cells spread. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:275 / 281
页数:7
相关论文
共 38 条
[1]   Modification of polysiloxane polymers for biomedical applications: a review [J].
Abbasi, F ;
Mirzadeh, H ;
Katbab, AA .
POLYMER INTERNATIONAL, 2001, 50 (12) :1279-1287
[2]   Exploiting the current paradigm of blood-material interactions for the rational design of blood-compatible materials [J].
Brash, JL .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2000, 11 (11) :1135-1146
[3]   Does the nanometre scale topography of titanium influence protein adsorption and cell proliferation? [J].
Cai, Kaiyong ;
Bossert, Jorg ;
Jandt, Klaus D. .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2006, 49 (02) :136-144
[4]   Surface patterning: Tool to modulate stem cell differentiation in an adipose system [J].
Chaubey, Aditya ;
Ross, Kevin J. ;
Leadbetter, Ross M. ;
Burg, Karen J. L. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2008, 84B (01) :70-78
[5]   Biocompatible polymer materials: Role of protein-surface interactions [J].
Chen, Hong ;
Yuan, Lin ;
Song, Wei ;
Wu, Zhongkui ;
Li, Dan .
PROGRESS IN POLYMER SCIENCE, 2008, 33 (11) :1059-1087
[6]   Role of fibrinogen conformation in platelet activation [J].
Chiumiento, A. ;
Lamponi, S. ;
Barbucci, R. .
BIOMACROMOLECULES, 2007, 8 (02) :523-531
[7]   Effect of laser modified surface microtopochemistry on endothelial cell growth [J].
Duncan, A. C. ;
Rouais, F. ;
Lazare, S. ;
Bordenave, L. ;
Baquey, Ch. .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2007, 54 (02) :150-159
[8]   Adsorption of fibrinogen and lysozyme on silicon grafted with poly(2-methacryloyloxyethyl phosphorylcholine) via surface-initiated atom transfer radical polymerization [J].
Feng, W ;
Zhu, SP ;
Ishihara, K ;
Brash, JL .
LANGMUIR, 2005, 21 (13) :5980-5987
[9]   Protein adsorption on topographically nanostructured titanium [J].
Galli, C ;
Coen, MC ;
Hauert, R ;
Katanaev, VL ;
Wymann, MP ;
Gröning, P ;
Schlapbach, L .
SURFACE SCIENCE, 2001, 474 (1-3) :L180-L184
[10]   The dry-style antifogging properties of mosquito compound eyes and artificial analogues prepared by soft lithography [J].
Gao, Xuefeng ;
Yan, Xin ;
Yao, Xi ;
Xu, Liang ;
Zhang, Kai ;
Zhang, Junhu ;
Yang, Bai ;
Jiang, Lei .
ADVANCED MATERIALS, 2007, 19 (17) :2213-+