Nanoprobe-based genetic testing

被引：4

作者：

Zu, Yanbing ^{[1
]}

Tan, Min-Han ^{[1
]}

Chowbay, Balram ^{[2
,3
,4
]}

Lee, Soo Chin ^{[5
,6
]}

Yap, Huiling ^{[5
]}

Lee, Ming Ta Michael ^{[7
,8
]}

Lu, Liang-Suei ^{[7
]}

Chang, Chun-Ping ^{[7
]}

Ying, Jackie Y. ^{[1
]}

机构：

[1] Inst Bioengn & Nanotechnol, Singapore 138669, Singapore

[2] Natl Canc Ctr Singapore, Clin Pharmacol Lab, Singapore 169610, Singapore

[3] Duke NUS Grad Med Sch Singapore, Singapore, Singapore

[4] SingHlth Clin Pharmacol Core, Singapore, Singapore

[5] Natl Univ Hlth Syst, Natl Univ Canc Inst, Dept Haematol Oncol, Singapore, Singapore

[6] Canc Sci Inst, Singapore, Singapore

[7] Acad Sinica, Inst Biomed Sci, Taipei 115, Taiwan

[8] RIKEN, Ctr Integrat Med Sci, Core Genom Med, Lab Int Alliance Genom Res, Yokohama, Kanagawa, Japan

来源：

NANO TODAY | 2014年 / 9卷 / 02期

关键词：

Nanoparticle; Morpholino; Genotyping; Warfarin; Pharmacogenomics; WARFARIN; PHARMACOGENOMICS;

D O I：

10.1016/j.nantod.2014.04.003

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Plasmonic nanoprobes functionalized with morpholino oligos have been developed for genotyping of single-nucleotide polymorphisms (SNPs) based on calorimetric signals that could be easily visualized. The assay was conducted with a standard thermal cycler that would be available in any molecular diagnostic laboratory, facilitating assay deployment and allowing for cost-effective genetic testing with rapid turnaround time. Specifically, a warfarin sensitivity testing kit gauging three SNPs has been created and validated with 249 human genomic DNA samples in four independent institutes, demonstrating the robustness of our new platform. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：166 / 171

页数：6

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