Isolation and characterization of two DREB1 genes encoding dehydration-responsive element binding proteins in chicory (Cichorium intybus)

被引：15

作者：

Liang, Mingxiang ^{[1
,2
]}

Chen, Dandan ^{[1
,2
]}

Lin, Manman ^{[1
,2
]}

Zheng, Qingsong ^{[1
,2
]}

Huang, Zengrong ^{[1
,2
]}

Lin, Zhongyuan ^{[1
,2
]}

Zhao, Gengmao ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Key Lab Marine Biol, Nanjing, Jiangsu, Peoples R China

来源：

PLANT GROWTH REGULATION | 2014年 / 73卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Abiotic stresses; Subcellular localization; Yeast one-hybrid; TRANSCRIPTION FACTORS; SIGNAL-TRANSDUCTION; FUNCTIONAL-ANALYSIS; DROUGHT STRESSES; ERF/AP2; DOMAIN; HIGH-SALT; COLD; EXPRESSION; TOLERANCE; DNA;

D O I：

10.1007/s10725-013-9866-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two novel DREB (dehydration-responsive element-binding protein) genes, designated as CiDREB1A and CiDREB1B, were cloned from chicory (Cichorium intybus). Both of these genes contained a conserved EREBP/AP2 domain and were classified into the A-1 subgroup of the DREB subfamily based on phylogenetic analysis. Quantitative real-time PCR analysis revealed that low temperature, but not ABA, greatly induced the expression of both CiDREB1 genes, suggesting that these genes are involved in ABA-independent stress signaling pathways. A subcellular localization assay revealed that both CiDREBs localized to the nucleus. In addition, we showed by yeast one-hybrid analysis that these two CiDREB proteins bind to the DRE motif of RD19A. These results suggest that CiDREB1A and CiDREB1B are important regulators of stress-responsive signaling in chicory.

引用

页码：45 / 55

页数：11

共 27 条

[21] Dehydration-Responsive Element Binding Protein 1C, 1E, and 1G Promote Stress Tolerance to Chilling, Heat, Drought, and Salt in Rice [J].

Wang, Huanhuan ;

Lu, Shan ;

Guan, Xiangyu ;

Jiang, Yuan ;

Wang, Bin ;

Hua, Jian ;

Zou, Baohong .

FRONTIERS IN PLANT SCIENCE, 2022, 13

[22] Isolation and characterization of two ABRE-binding proteins: EABF and EABF1 from the oil palm [J].

Omidvar, Vahid ;

Abdullah, Siti Nor Akmar ;

Ho, Chai Ling ;

Mahmood, Maziah ;

Al-Shanfari, Ahmed Bakhit .

MOLECULAR BIOLOGY REPORTS, 2012, 39 (09) :8907-8918

[23] The cotton dehydration-responsive element binding protein GhDBP1 contains an EAR-motif and is involved in the defense response of Arabidopsis to salinity stress [J].

Dong, Chun-Juan ;

Huang, Bo ;

Liu, Jin-Yuan .

FUNCTIONAL PLANT BIOLOGY, 2010, 37 (01) :64-73

[24] Isolation and identification of two genes encoding leucine-rich repeat (LRR) proteins differentially responsive to pathogen attack and salt stress in tobacco [J].

Xu, Zhao-Shi ;

Xiong, Teng-Fei ;

Ni, Zhi-Yong ;

Chen, Xue-Ping ;

Chen, Ming ;

Li, Lian-Cheng ;

Gao, Dong-Yao ;

Yu, Xiu-Dao ;

Liu, Pei ;

Ma, You-Zhi .

PLANT SCIENCE, 2009, 176 (01) :38-45

[25] Dehydration-Responsive Element-Binding Factor 2C Activates PHOSPHATE TRANSPORTER1;9 in Arabidopsis thaliana in Response to Phosphate Starvation [J].

Lee, Jae Hyeok ;

Lee, Yeong Min ;

Kang, Chang Ho ;

Lim, Chae Oh .

JOURNAL OF PLANT BIOLOGY, 2025, 68 (02) :145-154

[26] Immunoproteomic and Two-Dimensional Difference Gel Electrophoresis Analysis of Arabidopsis Dehydration Response Element-Binding Protein 1A (DREB1A)-Transgenic Potato [J].

Nakamura, Rika ;

Satoh, Rie ;

Nakamura, Ryosuke ;

Shimazaki, Takayoshi ;

Kasuga, Mie ;

Yamaguchi-Shinozaki, Kazuko ;

Kikuchi, Akira ;

Watanabe, Kazuo N. ;

Teshima, Reiko .

BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2010, 33 (08) :1418-1425

[27] Isolation and characterization of two ABRE-binding proteins: EABF and EABF1 from the oil palm [J].

Vahid Omidvar ;

Siti Nor Akmar Abdullah ;

Chai Ling Ho ;

Maziah Mahmood ;

Ahmed Bakhit Al-Shanfari .

Molecular Biology Reports, 2012, 39 :8907-8918

← 1 2 3 →