Isolation and characterization of two DREB1 genes encoding dehydration-responsive element binding proteins in chicory (Cichorium intybus)

被引：15

作者：

Liang, Mingxiang ^{[1
,2
]}

Chen, Dandan ^{[1
,2
]}

Lin, Manman ^{[1
,2
]}

Zheng, Qingsong ^{[1
,2
]}

Huang, Zengrong ^{[1
,2
]}

Lin, Zhongyuan ^{[1
,2
]}

Zhao, Gengmao ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Key Lab Marine Biol, Nanjing, Jiangsu, Peoples R China

来源：

PLANT GROWTH REGULATION | 2014年 / 73卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Abiotic stresses; Subcellular localization; Yeast one-hybrid; TRANSCRIPTION FACTORS; SIGNAL-TRANSDUCTION; FUNCTIONAL-ANALYSIS; DROUGHT STRESSES; ERF/AP2; DOMAIN; HIGH-SALT; COLD; EXPRESSION; TOLERANCE; DNA;

D O I：

10.1007/s10725-013-9866-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two novel DREB (dehydration-responsive element-binding protein) genes, designated as CiDREB1A and CiDREB1B, were cloned from chicory (Cichorium intybus). Both of these genes contained a conserved EREBP/AP2 domain and were classified into the A-1 subgroup of the DREB subfamily based on phylogenetic analysis. Quantitative real-time PCR analysis revealed that low temperature, but not ABA, greatly induced the expression of both CiDREB1 genes, suggesting that these genes are involved in ABA-independent stress signaling pathways. A subcellular localization assay revealed that both CiDREBs localized to the nucleus. In addition, we showed by yeast one-hybrid analysis that these two CiDREB proteins bind to the DRE motif of RD19A. These results suggest that CiDREB1A and CiDREB1B are important regulators of stress-responsive signaling in chicory.

引用

页码：45 / 55

页数：11

共 39 条

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