Isolation and characterization of a human heart cDNA encoding a new member of the small heat shock protein family - HSPL27

被引：23

作者：

Lam, WY ^{[1
]}

Tsui, SKW ^{[1
]}

Law, PTW ^{[1
]}

Luk, SCW ^{[1
]}

Fung, KP ^{[1
]}

Lee, CY ^{[1
]}

Waye, MMY ^{[1
]}

机构：

[1] CHINESE UNIV HONG KONG, DEPT BIOCHEM, SHATIN, NT, HONG KONG

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1996年 / 1314卷 / 1-2期

关键词：

small-heat-shock-protein; heart cDNA; chromosome; 5; (human);

D O I：

10.1016/S0167-4889(96)00121-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel cDNA clone was isolated rom a human adult heart cDNA library. This cDNA clone is similar to the small heat shock protein (smhsp) in both DNA and amino acid sequences, especially in the conserved region. Sequence analysis has shown that the putative novel smhsp. named 27 kDa heat-shock-protein-like protein (HSPL27) is a protein of 241 amino acids with a deduced molecular mass of 26.7 kDa and a deduced pi of 8.0. We have expresed the HSPL27 in E. coli and the expressed protein was found to be present in the soluble fraction of the bacterial cell lysate. Chromosomal mapping data shows that the HSPL27 gene is located ar human chromosome 5q11.2.

引用

页码：120 / 124

页数：5

共 32 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] THE GENES ENCODING NK-CELL GRANULE SERINE PROTEASES, HUMAN TRYPTASE-2 (TRYP2) AND HUMAN GRANZYME-A (HFSP), BOTH MAP TO CHROMOSOME-5Q11-Q12 AND DEFINE A NEW LOCUS FOR CYTOTOXIC LYMPHOCYTE GRANULE TRYPTASES [J].

BAKER, E ;

SAYERS, TJ ;

SUTHERLAND, GR ;

SMYTH, MJ .

IMMUNOGENETICS, 1994, 40 (03) :235-237

[3]

BASSETT AS, 1988, LANCET, V1, P799

[4] TRANSGLUTAMINASE IS ESSENTIAL IN RECEPTOR-MEDIATED ENDOCYTOSIS OF ALPHA-2-MACROGLOBULIN AND POLYPEPTIDE HORMONES [J].

DAVIES, PJA ;

DAVIES, DR ;

LEVITZKI, A ;

MAXFIELD, FR ;

MILHAUD, P ;

WILLINGHAM, MC ;

PASTAN, IH .

NATURE, 1980, 283 (5743) :162-167

[5] INDUCTION AND ACTIVATION OF TISSUE TRANSGLUTAMINASE DURING PROGRAMMED CELL-DEATH [J].

FESUS, L ;

THOMAZY, V ;

FALUS, A .

FEBS LETTERS, 1987, 224 (01) :104-108

[6] DE-NOVO APPARENTLY BALANCED RECIPROCAL TRANSLOCATION BETWEEN 5Q11.2 AND 17Q23 ASSOCIATED WITH KLIPPEL-FEIL ANOMALY AND TYPE A1 BRACHYDACTYLY [J].

FUKUSHIMA, Y ;

OHASHI, H ;

WAKUI, K ;

NISHIMOTO, H ;

SATO, M ;

AIHARA, T .

AMERICAN JOURNAL OF MEDICAL GENETICS, 1995, 57 (03) :447-449

[7] ASSIGNMENT OF THE HUMAN DIHYDROFOLATE-REDUCTASE GENE TO THE Q11-]Q22 REGION OF CHROMOSOME-5 [J].

FUNANAGE, VL ;

MYODA, TT ;

MOSES, PA ;

COWELL, HR .

MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (10) :2010-2016

[8]

GAESTEL M, 1991, J BIOL CHEM, V266, P14721

[9] ISCHEMIA AND THE MYOCYTE CYTOSKELETON - REVIEW AND SPECULATION [J].

GANOTE, C ;

ARMSTRONG, S .

CARDIOVASCULAR RESEARCH, 1993, 27 (08) :1387-1403

[10] MODES OF DAPI BANDING AND SIMULTANEOUS INSITU HYBRIDIZATION [J].

HENG, HHQ ;

TSUI, LC .

CHROMOSOMA, 1993, 102 (05) :325-332

← 1 2 3 4 →