Adipose-derived adult stromal cells heal critical-size mouse calvarial defects

被引：737

作者：

Cowan, CM

Shi, YY

Aalami, OO

Chou, YF

Mari, C

Thomas, R

Quarto, N

Contag, CH

Wu, B

Longaker, MT

机构：

[1] Stanford Univ, Sch Med, Dept Surg, Stanford, CA 94305 USA

[2] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA USA

[3] Stanford Univ, Sch Med, Dept Radiol, Div Nucl Med, Stanford, CA 94305 USA

[4] Stanford Univ, Sch Med, Dept Pediat, Stanford, CA 94305 USA

来源：

NATURE BIOTECHNOLOGY | 2004年 / 22卷 / 05期

关键词：

D O I：

10.1038/nbt958

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In adults and children over two years of age, large cranial defects do not reossify successfully, posing a substantial biomedical burden. The osteogenic potential of bone marrow stromal (BMS) cells has been documented. This study investigates the in vivo osteogenic capability of adipose-derived adult stromal (ADAS) cells, BMS cells, calvarial-derived osteoblasts and dura mater cells to heal critical-size mouse calvarial defects. Implanted, apatite-coated, PLGA scaffolds seeded with ADAS or BMS cells produced significant intramembranous bone formation by 2 weeks and areas of complete bony bridging by 12 weeks as shown by X-ray analysis, histology and live micromolecular imaging. The contribution of implanted cells to new bone formation was 84-99% by chromosomal detection. These data show that ADAS cells heal critical-size skeletal defects without genetic manipulation or the addition of exogenous growth factors.

引用

页码：560 / 567

页数：8

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