Cell-permeable fluorescent indicator for imaging formaldehyde activity in living systems

被引：5

作者：

Liu, Jun ^{[1
]}

Li, Kaipeng ^{[1
]}

Xue, Peng ^{[1
]}

Xu, Jinyi ^{[1
]}

机构：

[1] Hexi Univ, Coll Chem & Chem Engn, Zhangye 734000, Gansu, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2022年 / 652卷

关键词：

Cell permeability; Formaldehyde; Cancerous cells; Tumors; Probe; ENDOGENOUS FORMALDEHYDE; FLUOROGENIC PROBE;

D O I：

10.1016/j.ab.2022.114749

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Formaldehyde (FA), as a reactive signaling molecule, plays an important role in living systems through a diverse array of cellular pathways. However, no systematic investigation for detection and imaging of FA by rendering cells transiently permeable has been reported yet. Specifically, we developed a new cell-permeable fluorescence probe functionality that was enhanced cellular entry efficiency and well retained intracellularly after activation for visualizing endogenous FA changes. Moreover, a smart "multi-lock system -key-and-lock" strategy,which have provoked a starting point for the use of probe and related biochemical tools to monitor FA in lysosomes. The versatile "latent" fluorophore that can undergo a subsequent self-immolative spacer for interrogating the roles and functions of FA in living systems as well as related biomedical applications.

引用

页数：6

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