Growth characteristic and ion contents of rice callus under the influence of NaCl and hydroxyproline

Calli of salt tolerant (Bhoora rata) and salt susceptible (GR(11)) rice varieties were cultured on Linsmaeir and Skoog's medium containing LD50 concentration of NaCl (200 mM) and hydroxyproline (10 mM). Growth rate of callus and Na+, K+, Cl-, Mg+2, and Ca+2 contents of the cultured rice tissues were determined at the end of 0, 2, 4 and 6 weeks of incubation. Hydroxyproline: resistant calli of both rice varieties when cultured on Linsmaeir and Skoog's medium containing both NaCl and hydroxyproline showed increased dry weight and enhanced intracellular levels of K+, Mg+2 and Ca+2. The accumulation of Na+ and Cl- ions was less in the hydroxyproline resistant calli.