An integrated Ulva-periphyton biofilter for mariculture effluents: Multiple nitrogen removal kinetics

Biofilters made of Ulva and periphyton differ in their effectiveness in removing ammonia and nitrate from mariculture effluents. Our research evaluated the practicality of a combination of these two biofilters in improving the overall removal of dissolved N, where efficient removal of ammonia by the seaweed is followed by efficient removal of nitrate by periphyton. A paired Ulva-periphyton biofilter was exposed to various areal loads of ammonia and nitrate, the primary nitrogen forms in fishpond effluents. A first upstream macroalgae biofilter stocked with Ulva was fed with fishpond effluents at different areal loads of ammonia and nitrate, while a second downstream periphyton biofilter was paired for further nitrogen removal from the effluent. Ulva removed ammonia at a rate of 0.7-5.4 g TAN m(-2) d(-1), in correlation with the TAN areal load, with V-max of 5.1 and K-m of 4.4 g TAN m(-2) d(-1). Downstream periphyton was exposed to a lower TAN, but nitrate-rich effluent, and revealed similar capacities for the removal of both N forms, at removal rates of up to 1.7 and 1.8 g Nm(-2) d(-1), respectively. Compared to nitrate, areal load of TAN had a greater impact on the removal dynamics of both N forms by periphyton. Overall, the paired biofilter resulted in a nearly total depletion of ammonia (97%) and efficient nitrate removal (67%), when areal loads in fishpond effluents were below 2 and 4 g Nm(-2) d(-1) of TAN and NO3-N, respectively.