Neuroprotective effects of lycopene pretreatment on transient global cerebral ischemia-reperfusion in rats: The role of the Nrf2/HO-1 signaling pathway

被引：57

作者：

Lei, Xiaofeng ^{[1
]}

Lei, Lijian ^{[2
]}

Zhang, Zhelin ^{[1
]}

Cheng, Yan ^{[1
]}

机构：

[1] Tianjin Fourth Ctr Hosp, Dept Neurol, Tianjin 300140, Peoples R China

[2] Shanxi Med Univ, Dept Teaching & Res, Epidemiol Sect, Taiyuan 030001, Shanxi, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2016年 / 13卷 / 01期

关键词：

lycopene; apoptosis; global cerebral ischemia; Nrf2/HO-1; OXIDATIVE STRESS; DEFENSE PATHWAY; ISCHEMIA/REPERFUSION-INJURY; ALZHEIMERS-DISEASE; ANTIOXIDANT; APOPTOSIS; INDUCTION; PROTECTS; DAMAGE; CELLS;

D O I：

10.3892/mmr.2015.4534

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The present study aimed to investigate the neuroprotective effect of lycopene in a mouse model of bilateral common carotid artery occlusion (BCCAO) and the role of the Nrf2/HO-1 signaling pathway. A total of 60 male C57BL/6 mice, aged 12 weeks and weighing 20-24 g, were used in the present study. The mice were randomly assigned to three groups: Control, BCCAO and BCCAO + lycopene. The neurological score was assessed 24, 48 or 72 h following BCCAO. Hematoxylin and eosin staining, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were performed to detect neuronal death and survival. The production of glutathione (GSH) and reactive oxygen species were detected to investigate the oxidative stress. The expression levels of nuclear factor erythroid 2-related factor (Nrf2) and Heme oxygenase-1 (HO-1) were determined by western blotting. Lycopene significantly improved the neurological score in the BCCAO mice. It attenuated neuronal apoptosis, as indicated by TUNEL staining, and attenuated the oxidative stress induced by global ischemia. Lycopene increased the expression levels of Nrf2 and HO-1, indicating that the Nrf2/HO-1 signaling pathway may be involved in the neuroprotective effect of lycopene. The present study revealed that lycopene protects the brain from global ischemic injury, which is associated with its antiapoptotic effect and the activation of the Nrf2/HO-1 signaling pathway.

引用

页码：412 / 418

页数：7

共 43 条

[1] Targeting the Nrf2-Keap1 antioxidant defence pathway for neurovascular protection in stroke [J].

Alfieri, Alessio ;

Srivastava, Salil ;

Siow, Richard C. M. ;

Modo, Michel ;

Fraser, Paul A. ;

Mann, Giovanni E. .

JOURNAL OF PHYSIOLOGY-LONDON, 2011, 589 (17) :4125-4136

[2] CHARACTERISTICS OF TRANSIENT CEREBRAL ISCHEMIA-INDUCED DEFICITS ON VARIOUS LEARNING AND MEMORY TASKS IN MALE MONGOLIAN GERBILS [J].

AMANO, M ;

HASEGAWA, M ;

HASEGAWA, T ;

NABESHIMA, T .

JAPANESE JOURNAL OF PHARMACOLOGY, 1993, 63 (04) :469-477

[3] Signaling, delivery and age as emerging issues in the benefit/risk ratio outcome of tPA For treatment of CNS ischemic disorders [J].

Armstead, William M. ;

Ganguly, Kumkum ;

Kiessling, J. W. ;

Riley, John ;

Chen, Xiao-Han ;

Smith, Douglas H. ;

Stein, Sherman C. ;

Higazi, Abd A. R. ;

Cines, Douglas B. ;

Bdeir, Khalil ;

Zaitsev, Sergei ;

Muzykantov, Vladimir R. .

JOURNAL OF NEUROCHEMISTRY, 2010, 113 (02) :303-312

[4] The complexity of neurobiological processes in acute ischemic stroke [J].

Brouns, R. ;

De Deyn, P. P. .

CLINICAL NEUROLOGY AND NEUROSURGERY, 2009, 111 (06) :483-495

[5] A Combination of Supplements May Reduce the Risk of Alzheimer's Disease in Elderly Japanese with Normal Cognition [J].

Bun, Shogyoku ;

Ikejima, Chiaki ;

Kida, Jiro ;

Yoshimura, Atsuko ;

Lebowitz, Adam Jon ;

Kakuma, Tatsuyuki ;

Asada, Takashi .

JOURNAL OF ALZHEIMERS DISEASE, 2015, 45 (01) :15-25

[6] Lycopene protects against apoptosis in hypoxia/reoxygenation-induced H9C2 myocardioblast cells through increased autophagy [J].

Chen, Fei ;

Sun, Ze-Wei ;

Ye, Li-Fang ;

Fu, Guo-Sheng ;

Mou, Yun ;

Hu, Shen-Jiang .

MOLECULAR MEDICINE REPORTS, 2015, 11 (02) :1358-1365

[7] Oxidative Stress in Ischemic Brain Damage: Mechanisms of Cell Death and Potential Molecular Targets for Neuroprotection [J].

Chen, Hai ;

Yoshioka, Hideyuki ;

Kim, Gab Seok ;

Jung, Joo Eun ;

Okami, Nobuya ;

Sakata, Hiroyuki ;

Maier, Carolina M. ;

Narasimhan, Purnima ;

Goeders, Christina E. ;

Chan, Pak H. .

ANTIOXIDANTS & REDOX SIGNALING, 2011, 14 (08) :1505-1517

[8] Initiator caspases in apoptosis signaling pathways [J].

Chen, M ;

Wang, J .

APOPTOSIS, 2002, 7 (04) :313-319

[9] Impaired Redox Signaling and Antioxidant Gene Expression in Endothelial Cells in Diabetes: A Role for Mitochondria and the Nuclear Factor-E2-Related Factor 2-Kelch-Like ECH-Associated Protein 1 Defense Pathway [J].

Cheng, Xinghua ;

Siow, Richard C. M. ;

Mann, Giovanni E. .

ANTIOXIDANTS & REDOX SIGNALING, 2011, 14 (03) :469-487

[10]

Ciftci O, 2014, EUR REV MED PHARMACO, V18, P3320

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