From implantation to degradation - are poly (L-lactide)/multiwall carbon nanotube composite materials really cytocompatible?

被引:36
作者
Obarzanek-Fojt, Magdalena [1 ]
Elbs-Glatz, Yvonne [1 ]
Lizundia, Erlantz [2 ,3 ]
Diener, Liliane [1 ]
Sarasua, Jose-Ramon [2 ,3 ]
Bruinink, Arie [1 ]
机构
[1] Empa, Mat Biol Interact, St Gallen, Switzerland
[2] Univ Basque Country UPV EHU, Dept Min Met Engn & Mat Sci, Bilbao, Spain
[3] Univ Basque Country UPV EHU, BERC POLYMAT, Bilbao, Spain
关键词
Polylactic acid; MWCNT; Biocompatibility; Bone regeneration; Cytotoxicity; IN-VITRO; LACTIC-ACID; CELLS; BIOCOMPATIBILITY; NANOCOMPOSITES; CYTOTOXICITY; FIXATION; VIVO; DIFFERENTIATION; POLY(L-LACTIDE);
D O I
10.1016/j.nano.2013.12.012
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Poly (L-lactide)'s (PLLA) biodegradable properties are of special value in orthopaedic applications, but its mechanical strength limits its usage. To overcome this PLLA can be reinforced by multiwall carbon nanotubes (MWCNT). In this study the PLLA and MWCNT were combined to prepare nanostructured composites (nanocomposite) at 0, 0.1, 0.5 and 1 wt.% reinforcement. The in vitro biocompatibility of these PLLA/MWCNT nanocomposites was evaluated taking into account the various stages of implantation including nanocomposite degradation. PLLA/MWCNT nanocomposites were highly biocompatible with human bone marrow stromal cells (HBMC). The potential surface degradation product, MWCNT, did not induce toxic responses on HBMC. However, the combination of MWCNT with lactic acid, resembling release after bulk degradation, significantly inhibited HBMC proliferation and activity. This study demonstrates the importance of comprehensive evaluations of novel materials for medical applications in predicting possible adverse effects during nanocomposite degradation. From the Clinical Editor: This study scrutinizes the cytocompatibility of poly-L-lactide reinforced by multiwall carbon nanotubes, and concludes that the combination of MWCNT with lactic acid significantly inhibited human bone marrow stromal cell proliferation and activity, highlighting the importance of comprehensive evaluations of novel materials. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:1041 / 1051
页数:11
相关论文
共 45 条
[1]   Sterilization, toxicity, biocompatibility and clinical applications of polylactic acid polyglycolic acid copolymers [J].
Athanasiou, KA ;
Niederauer, GG ;
Agrawal, CM .
BIOMATERIALS, 1996, 17 (02) :93-102
[2]   Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging [J].
Avti, Pramod K. ;
Sitharaman, Balaji .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 :1953-1964
[3]  
Barca F, 1997, J Foot Ankle Surg, V36, P106
[4]  
Barca F, 1997, J FOOT ANKLE SURG, V36, P60
[5]   Pluronic-coated carbon nanotubes do not induce degeneration of cortical neurons in vivo and in vitro [J].
Bardi, Giuseppe ;
Tognini, Paola ;
Ciofani, Gianni ;
Raffa, Vittoria ;
Costa, Mario ;
Pizzorusso, Tommaso .
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2009, 5 (01) :96-104
[6]  
BRUNON J, 1994, NEUROCHIRURGIE, V40, P196
[7]  
Burns A E, 1998, J Foot Ankle Surg, V37, P37
[8]   Effects of different treatments on the interface of HDPE/lignocellulosic fiber composites [J].
Colom, X ;
Carrasco, F ;
Pagès, P ;
Cañavate, J .
COMPOSITES SCIENCE AND TECHNOLOGY, 2003, 63 (02) :161-169
[9]   Lactic Acid and Acidification Inhibit TNF Secretion and Glycolysis of Human Monocytes [J].
Dietl, Katrin ;
Renner, Kathrin ;
Dettmer, Katja ;
Timischl, Birgit ;
Eberhart, Karin ;
Dorn, Christoph ;
Hellerbrand, Claus ;
Kastenberger, Michael ;
Kunz-Schughart, Leoni A. ;
Oefner, Peter J. ;
Andreesen, Reinhard ;
Gottfried, Eva ;
Kreutz, Marina P. .
JOURNAL OF IMMUNOLOGY, 2010, 184 (03) :1200-1209
[10]   Uptake and cytotoxic effects of multi-walled carbon nanotubes in human bronchial epithelial cells [J].
Hirano, Seishiro ;
Fujitani, Yuji ;
Furuyama, Akiko ;
Kanno, Sanae .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2010, 249 (01) :8-15