Different reactivities of enzyme-linked immunosorbent assays for hepatocyte growth factor

被引:4
作者
Fukuta, Kazuhiro [1 ]
Adachi, Eri [1 ]
Matsumoto, Kunio [2 ]
Nakamura, Toshikazu [1 ]
机构
[1] Osaka Univ, Ctr Adv Sci & Innovat, Kringle Pharma Joint Res Div Regenerat Drug Disco, Suita, Osaka 5650871, Japan
[2] Kanazawa Univ, Canc Res Inst, Div Tumor Dynam & Regulat, Kanazawa, Ishikawa 9200934, Japan
关键词
HGF; ELISA; Reactivity; FACTOR/SCATTER FACTOR; HEPATECTOMIZED RATS; C-MET; LIVER; SERUM; PURIFICATION; EXPRESSION; INHIBITORS; CLEARANCE; RECEPTOR;
D O I
10.1016/j.cca.2008.12.011
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Hepatocyte growth factor (HGF) plays diverse roles in organ development, tissue regeneration, and tumor progression. Measurement of HGF concentrations in blood and tissues using enzyme-linked immunosorbent assay (ELISA) is a simple and easy way to understand the significance of HGF in tissue regeneration, pathophysiology, and diagnosis. Methods: We evaluated 3 ELISA kits from different sources, referred to herein as kits A, B, and C for convenience. Results: We found that the concentrations of human HGF determined using ELISA vary significantly depending on the source of the ELISA kit. Kits A and B detected both single-chain pro-HGF and 2-chain mature HGF, but kit C detected only 2-chain HGF. A difference in reactivity was also detected during analysis of plasma samples. When rat plasma collected 4 h after subcutaneous administration of human HGF was analyzed, the HGF concentration determined using kit B was remarkably higher than those obtained using kits A and C. Results of a biological assay and Western blot analysis indicated that kit B detects even degraded HGF, by which the HGF concentrations determined using kit B were significantly overestimated. Conclusions: This information serves as a guide for the selection of ELISA kits for human HGF. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:42 / 46
页数:5
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