Effect of chromium salts on invertase immobilization onto carboxymethylcellulose-gelatine carrier system

被引：21

作者：

Emregul, E

Sungur, S

Akbulut, U

机构：

[1] ANKARA UNIV,DEPT CHEM,ANKARA 06100,TURKEY

[2] MIDDLE E TECH UNIV,DEPT CHEM,ANKARA 06531,TURKEY

来源：

BIOMATERIALS | 1996年 / 17卷 / 14期

关键词：

gelatine; carboxymethylcellulose; invertase enzyme; immobilization;

D O I：

10.1016/0142-9612(96)87285-1

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The carboxymethylcellulose-gelatine carrier system was investigated for invertase immobilization. Chromium (III) acetate, chromium (III) sulphate and potassium chromium (III) sulphate were used as cross-linking agents. Effect of carboxymethylcellulose-gelatine ratio and cross-linker concentration on immobilized enzyme activity were analysed. Reusability of immobilized enzyme was also investigated. Maximum immobilized enzyme activities were obtained with cross-linkers chromium (III) sulphate (0.004 mol dm(-3)) and potassium chromium (III) sulphate (0.001 mol dm(-3)) for a carrier composition of carboxymethylcellulose-gelatine ratio 0.111 (w/w) as 78%. (C) 1996 Elsevier Science Limited

引用

页码：1423 / 1427

页数：5

共 9 条

[1]

Coughlin R. W., 1980, IMMOBILIZED ENZYMES, P135

[2] APPLICATION OF IMMOBILIZED INVERTASE TO CONTINUOUS HYDROLYSIS OF CONCENTRATED SUCROSE SOLUTIONS [J].