Aquaporin-4 Overexpression in Rat ALS Model

被引：55

作者：

Nicaise, Charles ^{[1
]}

Soyfoo, Muhammad Shahnawaz ^{[2
]}

Authelet, Michele ^{[1
]}

De Decker, Robert ^{[1
]}

Bataveljic, Danijela ^{[3
]}

Delporte, Christine ^{[2
]}

Pochet, Roland ^{[1
]}

机构：

[1] Univ Libre Bruxelles, Lab Histol Neuroanat & Neuropathol, B-1070 Brussels, Belgium

[2] Univ Libre Bruxelles, Lab Biol Chem & Nutr, B-1070 Brussels, Belgium

[3] Fac Biol, Inst Physiol & Biochem, Belgrade, Serbia

来源：

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2009年 / 292卷 / 02期

关键词：

aquaporin-4; amyotrophic lateral sclerosis; astrocyte end-feet; BLOOD-BRAIN-BARRIER; AMYOTROPHIC-LATERAL-SCLEROSIS; SPINAL-CORD; MESSENGER-RNA; WATER TRANSPORT; EXPRESSION; MICE; ASTROCYTES; CANDIDATE;

D O I：

10.1002/ar.20838

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Onset of motoneuron death characterizing amyotrophic lateral sclerosis (ALS) is closely linked to modified astrocytic and glial environments. Here, we show that in the spinal cord from transgenic rat overexpressing mutated human SOD1, aquaporin-4 mRNA and protein are specifically overexpressed in the gray matter at end stage of disease. Immunohistochemistry and double immunofluorescence allowed to detect, in the spinal cord gray matter of the ALS rat, increased aquaporin-4 surrounding both vessel and motoneuron perikarya. The use of pre-embedding immunohistochemistry at electron microscopic level confirmed such localization associated with swollen astrocytic processes surrounding the vessels. The AQP4 immunohistochemical labeling surrounding several motoneuron perikarya was only seen in ALS rats. Identification of this AQP4-positive cellular type remains unclear. Anat Rec, 292:207-213, 2009. (C) 2008 Wiley-Liss, Inc.

引用

页码：207 / 213

页数：7

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