Anti-Inflammatory Mechanism of Raddeanin A on RAW 264.7 Macrophage Inflammation Model Induced by LPS

Inflammation is a complex defensive response of organisms with vascular system to injury factors. Nonsteroidal antiinflammatory drugs currently used in clinic have a series of adverse reactions. Thus searching for non-steroidal antiinflammatory drugs with good anti-inflammatory activity and low adverse reactions has always been very important. The Rhizoma Anemones Raddeanae (RAR) is a kind of medicine for rheumatism treatment and applied clinically in various fields such as wind and cold symptoms, hand-paw and spasm, joint pain and ulcer pain. The proliferation effect of RDA worked on the RAW 264.7 cells was detected by MTT assay. Griess assay was used to detect the content of Nitric Oxide (NO). And the ELISA assay was used to detect the secretion of TNF-alpha, IL-6 and IL-1 beta. The changes of the protein contents of NF-kappa B p65, P-NF-kappa B p65, Akt, P-Akt, JNK, P-JNK in RAW 264.7 cells induced by LPS were detected by western blot assay. The RT-PCR assay was used to detect the changes of TNF-alpha, IL-6, IL-1beta, iNOS and COX-2 genes expression in the RAW 264.7 cells induced by LPS. RDA could play an anti-inflammatory effect by inhibiting NF-kappa B/MAPK and iNOS/COX-2 signal pathway. RDA could inhibit the proliferation of RAW 264.7 induced by LPS, effectively reduce the release of NO, and the anti-inflammatory activity of RDA was better. It could inhibit the secretion of TNF-alpha, IL-1 beta and IL-6, and showed a dose-dependent relationship. We found that RDA could inhibit the proliferation of RAW 264.7 induced by LPS, effectively reduce the release of NO, and the anti-inflammatory activity of RDA was better. It could inhibit the secretion of TNF-alpha, IL-1 beta and IL-6. (C) 2019 Friends Science Publishers