Hierarchical ZIF-8 toward Immobilizing Burkholderia cepacia Lipase for Application in Biodiesel Preparation

被引：66

作者：

Adnan, Miaad ^{[1
,2
]}

Li, Kai ^{[1
]}

Wang, Jianhua ^{[1
]}

Xu, Li ^{[1
]}

Yan, Yunjun ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Peoples R China

[2] Minist Sci & Technol, Baghdad 10001, Iraq

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 05期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

adsorption method; biodiesel production; Burkholderia cepacia lipase (BCL); hexahedral ZIF-8; hierarchical ZIF-8; surfactant; METAL-ORGANIC FRAMEWORK; RHIZOPUS-ORYZAE LIPASE; MIXED MATRIX MEMBRANE; CATALYZED TRANSESTERIFICATION; MAGNETIC NANOPARTICLES; ENZYME IMMOBILIZATION; OIL; NANOCOMPOSITE; ENCAPSULATION; PERFORMANCE;

D O I：

10.3390/ijms19051424

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A hierarchical mesoporous zeolitic imidazolate framework (ZIF-8) was processed based on cetyltrimethylammonium bromide (CTAB) as a morphological regulating agent and amino acid (l-histidine) as assisting template agent. Burkholderia cepacia lipase (BCL) was successfully immobilized by ZIF-8 as the carrier via an adsorption method (BCL-ZIF-8). The immobilized lipase (BCL) showed utmost activity recovery up to 1279%, a 12-fold boost in its free counterpart. BCL-ZIF-8 was used as a biocatalyst in the transesterification reaction for the production of biodiesel with 93.4% yield. There was no significant lowering of conversion yield relative to original activity for BCL-ZIF-8 when continuously reused for eight cycles. This work provides a new outlook for biotechnological importance by immobilizing lipase on the hybrid catalyst (ZIF-8) and opens the door for its uses in the industrial field.

引用

页数：14

共 61 条

[1]

Abdulla R., 2013, CROSS LINKED LIPASE, P197

[2] X-Shaped ZIF-8 for Immobilization Rhizomucor miehei Lipase via Encapsulation and Its Application toward Biodiesel Production [J].