Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates

被引：26

作者：

Gueixens-Gallardo, Pedro ^{[1
]}

Hocek, Michal ^{[1
,2
]}

Perlikova, Pavla ^{[1
]}

机构：

[1] Acad Sci Czech Republic, Inst Organ Chem & Biochem, Gilead & IOCB Res Ctr, CZ-16610 Prague 6, Czech Republic

[2] Charles Univ Prague, Fac Sci, Dept Organ Chem, CZ-12843 Prague 2, Czech Republic

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2016年 / 26卷 / 02期

关键词：

DNA polymerases; Nucleotide addition; Primer extension; Oligonucleotides; Twisted intercalating nucleic acid; ELECTROCHEMICAL DETECTION; SUGAR RECOGNITION; TRIPHOSPHATES; AMPLIFICATION; APTAZYMES; APTAMERS; ARCHAEON; BEARING; PCR; END;

D O I：

10.1016/j.bmcl.2015.12.034

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A simple and elegant method for inhibition of non-templated nucleotide addition by DNA polymerases and for following DNA 30-heterogeneity in enzymatic DNA synthesis by primer extension (PEX) is described. When template bearing ortho-twisted intercalating nucleic acid (ortho-TINA) at the 5'-end is used, non-templated nucleotide addition is reduced in both the A-and B-family DNA polymerases (KOD XL, KOD (exo-), Bst 2.0, Therminator, Deep Vent (exo-) and Taq). Formation of a single oligonucleotide product was observed with ortho-TINA modified template and KOD XL, KOD (exo-), Bst 2.0, Deep Vent (exo-) and Taq DNA polymerases. This approach can be applied to the synthesis of both unmodified and base-modified oligonucleotides. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：288 / 291

页数：4