Unexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids

被引：158
作者：
Fujita, Kyoko
Forsyth, Maria
MacFarlane, Douglas R. [1 ]
Reid, Robert W.
EIliott, Gloria D.
机构：
[1] Monash Univ, Sch Chem, Clayton, Vic 3800, Australia
[2] Monash Univ, Sch Phys & Mat Engn, Clayton, Vic, Australia
[3] Univ N Carolina, Dept Biol, Charlotte, NC 28223 USA
[4] Univ N Carolina, Dept Mech Engn & Engn Sci, Charlotte, NC 28223 USA
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 2006年 / 94卷 / 06期
关键词：
biocompatible ionic liquids; protein stability; activity; secondary structure; biopreservation;
D O I：
10.1002/bit.20928
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Proteins generally are only stable in vitro for short periods of time. This results in challenges during isolation and purification of recombinant proteins and reduces the shelf life of protein-based pharmaceuticals. Here we show that certain novel, biocompatible ionic liquids provide a stabilizing solvent for proteins, for example, cytochrome c, such that structure and activity are maintained even after 6 months of storage at room temperature. Normally, this protein would be rendered inactive after only 1 week in buffered aqueous solution. The effect of the ionic liquid solvent appears to be related to protection against hydrolysis. (c) 2006 Wiley Periodicals, Inc.
引用
收藏
页码：1209 / 1213
页数：5
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