Sharpea azabuensis: a ruminal bacterium that produces trans-11 intermediates from linoleic and linolenic acid

被引：7
|
作者：
Dewanckele, Lore [1 ]
Vlaeminck, Bruno [1 ,2 ]
Fievez, Veerle [1 ]
机构：
[1] Univ Ghent, Lab Anim Nutr & Anim Prod Qual Lanupro, Dept Anim Sci & Aquat Ecol, Campus Coupure,Block F,Coupure Links 653, B-9000 Ghent, Belgium
[2] Univ Ghent, Dept Biol, Res Grp Marine Biol, Campus Sterre,Krijgslaan 281-S8, B-9000 Ghent, Belgium
来源：
MICROBIOLOGY-SGM | 2019年 / 165卷 / 07期
关键词：
biohydrogenation; linoleic acid; linolenic acid; Sharpea azabuensis; FATTY-ACIDS; COMMUNITY COMPOSITION; BIOHYDROGENATION; METABOLISM; ISOMERS; RUMEN;
D O I：
10.1099/mic.0.000811
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
To investigate the metabolism of 18: 2n-6 and 18: 3n-3 by pure cultures of Sharpea azabuensis, two different strains ( RL 1 and ST18) were each incubated in the presence of 40 mu g ml(-1) 18: 2n-6 or 18: 3n-3. Pure cultures of Butyrivibrio fibrisolvens D1 and Butyrivibrio proteoclasticus P18 were included as control treatments. Similar to the metabolism of B. fibrisolvens, both S. azabuensis strains converted 18: 2n-6 or 18: 3n-3 to cis-9, trans-11 CLA or cis-9, trans-11, cis-15 CLnA, after which it was further reduced to trans-11 18: 1 or trans-11, cis-15 18: 2, respectively. B. proteoclasticus additionally reduced trans-11 18: 1 to 18: 0. Trans-11, cis-15 18: 2 was also further metabolized by B. proteoclasticus, although trans-11 18: 1 did not accumulate, and only minor amounts of 18: 0 were formed. The time frame of 18: 2n-6 and 18: 3n-3 biohydrogenation by S. azabuensis was comparable with B. fibrisolvens, indicating that S. azabuensis and B. fibrisolvens might be alternative biohydrogenators of 18: 2n-6 and 18: 3n-3 in the rumen.
引用
收藏
页码：772 / 778
页数：7
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