Image analysis of defocused single-molecule images for three-dimensional molecule orientation studies

被引：160

作者：

Patra, D ^{[1
]}

Gregor, I ^{[1
]}

Enderlein, J ^{[1
]}

机构：

[1] Forschungszentrum Julich, Inst Biol Informat Proc 1, D-52425 Julich, Germany

来源：

JOURNAL OF PHYSICAL CHEMISTRY A | 2004年 / 108卷 / 33期

关键词：

D O I：

10.1021/jp048188m

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

An efficient algorithm for pattern matching has been developed based on least-squares analysis of fitting a discrete set of master patterns against measured images. This algorithm has been applied to determine three-dimensional molecule orientations in defocused single-molecule images. The developed algorithm exploits the excellent agreement between electrodynamic calculations of single-molecule emission and experimentally measured images. The procedure is found to be reliable and simple and can be applied to any kind of pattern recognition where the patterns to be recognized are precisely known a priori. The procedure works well even for noisy and low-intensity signals as usually encountered in single-molecule experiments.

引用

页码：6836 / 6841

页数：6

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