Establishment of a Developmental Compartment Requires Interactions between Three Synergistic Cis-regulatory Modules

被引：19
作者：
Bieli, Dimitri [1 ]
Kanca, Oguz [1 ]
Requena, David [2 ,3 ]
Hamaratoglu, Fisun [4 ,5 ]
Gohl, Daryl
Schedl, Paul
Affolter, Markus [1 ]
Slattery, Matthew [6 ]
Mueller, Martin [1 ]
Estella, Carlos [2 ,3 ]
机构：
[1] Univ Basel, Biozentrum, Basel, Switzerland
[2] Univ Autonoma Madrid, Dept Biol Mol, Madrid, Spain
[3] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, Madrid, Spain
[4] Univ Lausanne, Ctr Integrat Genom, Lausanne, Switzerland
[5] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
[6] Univ Minnesota, Sch Med, Dept Biomed Sci, Duluth, MN 55812 USA
来源：
PLOS GENETICS | 2015年 / 11卷 / 10期
基金：
瑞士国家科学基金会;
关键词：
LONG-RANGE ACTION; LIM-HOMEODOMAIN ACTIVITY; GROUP RESPONSE ELEMENTS; DROSOPHILA WING DISC; GENE-EXPRESSION; DORSOVENTRAL COMPARTMENTALIZATION; TRANSCRIPTION FACTORS; MORPHOGEN GRADIENT; PATTERN-FORMATION; LEG DEVELOPMENT;
D O I：
10.1371/journal.pgen.1005376
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The subdivision of cell populations in compartments is a key event during animal development. In Drosophila, the gene apterous (ap) divides the wing imaginal disc in dorsal vs ventral cell lineages and is required for wing formation. ap function as a dorsal selector gene has been extensively studied. However, the regulation of its expression during wing development is poorly understood. In this study, we analyzed ap transcriptional regulation at the endogenous locus and identified three cis-regulatory modules (CRMs) essential for wing development. Only when the three CRMs are combined, robust ap expression is obtained. In addition, we genetically and molecularly analyzed the trans-factors that regulate these CRMs. Our results propose a three-step mechanism for the cell lineage compartment expression of ap that includes initial activation, positive autoregulation and Trithorax-mediated maintenance through separable CRMs.
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页数：30
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