Determination of quinolones in animal tissues and eggs by high-performance liquid chromatography with photodiode-array detection

被引：106

作者：

Gigosos, PG

Revesado, PR

Cadahía, O

Fente, CA ^{[1
]}

Vazquez, BI

Franco, CM

Cepeda, A

机构：

[1] Univ Santiago de Compostela, Fac Vet, Dept Quim Analit Nutr & Bromatol, Area Nutr & Bromatol, Lugo 27002, Spain

[2] Conselleria Sanidad & Serv Sociais, Lab Saude Publ, Lugo 27002, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 871卷 / 1-2期

关键词：

quinolones; antibiotics;

D O I：

10.1016/S0021-9673(99)01048-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, specific reversed-phase HPLC method is described, with solid-phase extraction, for assaying five quinolones (ciprofloxacin, difloxacin, enrofloxacin, norfloxacin and marbofloxacin) with confirmative diode-array detection in samples of bovine kidney, muscle and eggs. The least efficient extraction was marbofloxacin from kidney tissue (64%). The lower detection limit for each quinolone was: enrofloxacin and ciprofloxacin, 1 ng; norfloxacin and difloxacin, 2 ng; marbofloxacin, 4 ng injected. The intra-day relative standard deviations were lower than 7.9% and lower than 8.6% for inter-day assays. These results indicate that the developed method had an acceptable precision. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：31 / 36

页数：6

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