Curcumin-Induced DNA Damage and Inhibited DNA Repair Genes Expressions in Mouse-Rat Hybrid Retina Ganglion Cells (N18)

被引：35

作者：

Lu, Hsu-Feng ^{[2
]}

Yang, Jai-Sing ^{[3
]}

Lai, Kuang-Chi ^{[4
]}

Hsu, Shu-Chun ^{[5
]}

Hsueh, Shu-Ching ^{[2
]}

Chen, Yuan-Liang ^{[6
]}

Chiang, Jo-Hua ^{[7
]}

Lu, Chi-Cheng ^{[7
]}

Lo, Chyi ^{[8
]}

Yang, Mei-Due ^{[9
]}

Chung, Jing-Gung ^{[1
,10
]}

机构：

[1] China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan

[2] Cheng Hsin Rehabil Med Ctr, Dept Clin Pathol, Taipei, Taiwan

[3] China Med Univ, Dept Pharmacol, Taichung 404, Taiwan

[4] China Med Univ, Beigang Hosp, Dept Surg, Yunlin, Taiwan

[5] China Med Univ, Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan

[6] Yuan Pei Inst Sci & Technol, Dept Med Technol, Hsinchu, Taiwan

[7] Natl Chung Hsing Univ, Dept Life Sci, Taichung 40227, Taiwan

[8] China Med Univ, Grad Inst Chinese Med Sci, Sch Nursing, Taichung 404, Taiwan

[9] China Med Univ Hosp, Dept Surg, Taichung, Taiwan

[10] Asia Univ, Dept Biotechnol, Taichung, Taiwan

来源：

NEUROCHEMICAL RESEARCH | 2009年 / 34卷 / 08期

关键词：

Curcumin; DNA damage; DNA repair; N18 mouse-rat hybrid retina ganglion cells; HUMAN BREAST-CANCER; INDUCED APOPTOSIS; LIPID-PEROXIDATION; CRUDE EXTRACTS; NUCLEAR; MITOCHONDRIAL; CYTOTOXICITY; ACTIVATION; INDUCTION; ARREST;

D O I：

10.1007/s11064-009-9936-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Curcumin is reported to be a potent inhibitor of the initiation and promotion of many cancer cells. We investigated to examine whether or not curcumin induce DNA damage in mouse-rat hybrid retina ganglion cell line N18 cells. The Comet assay showed that incubation of N18 cells with 10, 25 and 30 mu M of curcumin led to a longer DNA migration smear (Comet tail). The DNA gel electrophoresis showed that 20 mu M of curcumin for 24 and 48 h treatment induced DNA damage and fragments in N18 cells. The real time PCR analysis showed that 20 mu M of curcumin for 48 h treatment decreased ATM, ATR, BRCA1, 14-3-3 sigma, DNA-PK and MGMT mRNA, and ATM and MGMT mRNA expression were inhibited in a time-dependent manner. Our results indicate that curcumin caused DNA damage and inhibited DNA repair genes which may be the factors for curcumin-inhibited cell growth.

引用

页码：1491 / 1497

页数：7

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