Liquid-chromatography-coupled SAXS for accurate sizing of aggregating proteins

被引：105

作者：

Mathew, E ^{[1
]}

Mirza, A ^{[1
]}

Menhart, N ^{[1
]}

机构：

[1] IIT, Chicago, IL 60616 USA

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2004年 / 11卷

关键词：

SAXS; liquid chromatography; Guinier; aggregation;

D O I：

10.1107/S0909049504014086

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X-rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results.

引用

页码：314 / 318

页数：5

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