Electrochemical methods for detection of post-translational modifications of proteins

被引:44
作者
Shumyantseva, Victoria V. [1 ]
Suprun, Elena V. [1 ]
Bulko, Tatiana V. [1 ]
Archakov, Alexander I. [1 ]
机构
[1] Russian Acad Med Sci, Inst Biomed Chem, Moscow 119121, Russia
关键词
Post-translational modifications; Electrochemistry; Phosphorylation; Biosensors; Glycosylation; BORON-DOPED DIAMOND; TYROSINE KINASE-ACTIVITY; GLASSY-CARBON ELECTRODE; AMINO-ACIDS; ELECTROCATALYTIC OXIDATION; GRAPHITE-ELECTRODES; MASS-SPECTROMETRY; SOLID ELECTRODES; S-NITROSYLATION; PASTE ELECTRODE;
D O I
10.1016/j.bios.2014.05.001
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Post-translational modifications of proteins play a key role in the regulation of various cellular processes. The analysis and identification of post-translational modifications are probably the most versatile and difficult, but also most frequently studied area of interest in proteomics research. This review focuses on the electroactivity of amino acids as a tool for analysis of post-translational modifications of proteins. The most attention is paid to the electrochemical detection of phosphorylation/dephosphorylation and glycosylation of proteins, to the best-studied and functionally-significant modifications, and, also, to the electrochemical analysis of activity of enzymes responsible for carrying out phosphorylation/dephosphorylation of proteins. Recent advances in electrochemistry with special references to proteomics are outlined and innovative technologies for protein detection are highlighted. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:131 / 139
页数:9
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