Rapid adaptation of avian H7N9 virus in pigs

被引：18
作者：
Xu, Lili [1 ,2 ]
Bao, Linlin [1 ,2 ]
Deng, Wei [1 ,2 ]
Zhu, Hua [1 ,2 ]
Li, Fengdi [1 ,2 ]
Chen, Ting [1 ,2 ]
Lv, Qi [1 ,2 ]
Yuan, Jing [1 ,2 ]
Xu, Yanfeng [1 ,2 ]
Li, Yanhong [1 ,2 ]
Yao, Yanfeng [1 ,2 ]
Gu, Songzhi [1 ,2 ]
Yu, Pin [1 ,2 ]
Chen, Honglin [3 ,4 ]
Qin, Chuan [1 ,2 ]
机构：
[1] CAMS, Inst Lab Anim Sci, Beijing, Peoples R China
[2] PUMC, Comparat Med Ctr, Key Lab Human Dis Comparat Med, Minist Hlth, Beijing, Peoples R China
[3] Univ Hong Kong, Dept Microbiol, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China
[4] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China
来源：
VIROLOGY | 2014年 / 452卷
基金：
中国国家自然科学基金;
关键词：
H7N9 influenza virus; Pig; Adaptation; Mutation; Receptor binding; INFLUENZA-A VIRUSES; IN-VIVO PRODUCTION; TRANSMISSION; INFECTION; ORIGIN;
D O I：
10.1016/j.virol.2014.01.016
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
How the H7N9 avian influenza virus gained the distinct ability to infect humans is unclear. Pigs are an important host in influenza virus ecology because they are susceptible to infection with both avian and human influenza viruses and are often involved in interspecies transmission. Here, we passaged one avian isolate and one human isolate in pigs to examine the mammalian host adaptation of the H7N9 virus. The avian virus replicated to a high titer after one passage, whereas the human isolate replicated poorly after three passages in pig lungs. Sequence analysis found nine substitutions in the HA, NA, M and NS segments of the avian isolate, which enhanced the binding affinity for human-type receptors. These results indicate that avian H7N9 influenza viruses can be easily adapted to pigs and that pigs may act as an important intermediate host for the reassortment and transmission of such novel viruses. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：231 / 236
页数：6
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