Inhibition of Long Non-coding RNA CTD-2574D22.4 Alleviates LPS-induced Apoptosis and Inflammatory Injury of Chondrocytes

被引:5
作者
Li, Lisong [1 ]
Zhang, Lianfang [1 ]
Zhang, Yong [1 ]
Jiang, Dinghua [1 ]
Xu, Wu [1 ]
Zhao, Haiyue [2 ]
Huang, Lixin [1 ]
机构
[1] Soochow Univ, Dept Orthoped Surg, Affiliated Hosp 1, 188 Shizi Rd, Suzhou 215006, Peoples R China
[2] Nanjing Med Univ, Suzhou Municipal Hosp, Ctr Reprod & Genet, Affiliated Suzhou Hosp, 26 Daoqian Rd, Suzhou 215002, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Osteoarthritis; LncRNA; CTD-2574D22.4; apoptosis; inflammatory injury; cartilage degeneration; LNCRNA TUG1; OSTEOARTHRITIS; EXPRESSION; CARTILAGE; MMP-13; DEGRADATION; INSIGHTS; MATRIX; DAMAGE; MODEL;
D O I
10.2174/1381612825666190801141801
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Background: Osteoarthritis (OA) is a common joint disease characterized by cartilage degeneration. Long non-coding RNAs (lncRNAs) have been associated with inflammatory diseases, including OA. Here, we investigated the potential molecular role of lncRNAs in OA pathogenesis. Methods: ATDC5 cells were treated with lipopolysaccharides (LPS), and ciPCR was used to identify and determine expression of potential lncRNAs involved in LPS-induced chondrocyte injury. Cell viability, apoptosis, and expression of cartilage-related genes and inflammatory cytokines were assessed after CTD-2574D22.4 knockdown. Results: After LPS stimulation, CTD-2574D22.4 was found to be the second highest up-regulated gene, and the enhanced expression was validated in OA chondrocytes. Moreover, CTD-2574D22.4 inhibition significantly rescued cell viability, suppressed by LPS stress. and markedly attenuated LPS-induced apoptosis. The expression of cartilage-degrading enzymes MtvIP-13 and ADAMTS-5 were increased, while type II collagen was reduced after LPS treatment. This trend was largely reversed by CTD-2574D22.4 knockdown. Additionally, mRNA and protein levels of key inflammatory cytokines (TNF-a, IL-6, and IL-1 beta) were significantly elevated in the LPS group and partially relieved upon CTD-2574D22.4 knockdown. Conclusion: CTD2574D22.4 knockdown ameliorates LPS-induced cartilage injury by protecting chondrocytes from apoptosis via anti-inflammation and anti- cartilage-degrading pathways. Thus, CTD2574D22.4 might be a potential diagnostic and therapeutic target for OA.
引用
收藏
页码:2969 / 2974
页数:6
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