A Comparison of Plasmid DNA and mRNA as Vaccine Technologies

被引：296
作者：
Liu, Margaret A. [1 ]
机构：
[1] ProTherImmune, 3656 Happy Valley Rd, Lafayette, CA 94549 USA
来源：
VACCINES | 2019年 / 7卷 / 02期
关键词：
DNA vaccine; mRNA vaccine; plasmid DNA; in vitro transcribed mRNA; immune responses; formulations; Cytolytic T Lymphocytes; antibodies; innate immunity; WEST-NILE-VIRUS; CYTOTOXIC T-LYMPHOCYTES; HEALTHY-ADULTS; INFLUENZA-VIRUS; INTRAMUSCULAR INJECTION; MITOCHONDRIAL TOXICITY; NEUTRALIZING ANTIBODY; RESPIRATORY-SYNDROME; IMMUNE-RESPONSES; OPEN-LABEL;
D O I：
10.3390/vaccines7020037
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
This review provides a comparison of the theoretical issues and experimental findings for plasmid DNA and mRNA vaccine technologies. While both have been under development since the 1990s, in recent years, significant excitement has turned to mRNA despite the licensure of several veterinary DNA vaccines. Both have required efforts to increase their potency either via manipulating the plasmid DNA and the mRNA directly or through the addition of adjuvants or immunomodulators as well as delivery systems and formulations. The greater inherent inflammatory nature of the mRNA vaccines is discussed for both its potential immunological utility for vaccines and for the potential toxicity. The status of the clinical trials of mRNA vaccines is described along with a comparison to DNA vaccines, specifically the immunogenicity of both licensed veterinary DNA vaccines and select DNA vaccine candidates in human clinical trials.
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页数：20
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