Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system

被引：6
|
作者：
Shan, Weiguang [2 ]
Li, Jiaping [2 ]
Fang, Ying [1 ]
Wang, Xuan [1 ]
Gu, Danxia [1 ]
Zhang, Rong [1 ]
机构：
[1] Zhejiang Univ, Affiliated Hosp 2, Hangzhou 310003, Zhejiang, Peoples R China
[2] Zhejiang Univ Technol, Coll Pharm Sci, Hangzhou, Zhejiang, Peoples R China
来源：
CURRENT MICROBIOLOGY | 2016年 / 72卷 / 01期
关键词：
FLIGHT MASS-SPECTROMETRY; DESORPTION IONIZATION-TIME; OUTBREAK;
D O I：
10.1007/s00284-015-0913-8
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A rapid, sensitive, and accurate Vitek MS assay was developed to distinguish clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) from clinical isolates of methicillin-sensitive Staphylococcus aureus (MSSA) by developing an in-house knowledgebase of SuperSpectra. Three unique peaks, including peaks at 2305.6 and 3007.3 Da specific to MRSA, and 6816.7 Da specific to MSSA, were selected for differentiating MRSA and MSSA. This assay accurately identified 84 and 91 % of clinical MRSA and MSSA strains out of the total 142 clinically acquired S. aureus strains that were tested. This method will greatly improve the efficiency of single clinical sample identification of MRSA, thereby facilitating a reduction in the transmission of MRSA in clinical settings.
引用
收藏
页码：29 / 32
页数：4
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