Chemical Characterization and Antimicrobial Activity Evaluation of Natural Oil Nanostructured Emulsions

被引：42
作者：
Alencar, Everton N. [1 ]
Xavier-Junior, Francisco H. [2 ,3 ]
Morais, Andreza R. V. [2 ]
Dantas, Teresa R. F. [2 ]
Dantas-Santos, Nednaldo [2 ]
Verissimo, Lourena M. [2 ]
Rehder, Vera L. G. [4 ]
Chaves, Guilherme M. [1 ]
Oliveira, Anselmo G. [5 ]
Egito, E. Socrates T. [1 ,2 ]
机构：
[1] Univ Fed Rio Grande Norte UFRN, CCS, PPGCF, BR-59010180 Natal, RN, Brazil
[2] Univ Fed Rio Grande Norte UFRN, CCS, UFRN, Fac Farm,Lab Sistemas Dispersos LaSiD, BR-59010180 Natal, RN, Brazil
[3] Univ Fed Rio Grande do Norte, Rede Nordeste Biotecnol RENORBIO, Programa Posgrad, BR-59000000 Natal, RN, Brazil
[4] Univ Estadual Campinas CAMP, Ctr Pluridisciplinar Pesquisas Quim, BR-13081970 Paulinia, SP, Brazil
[5] Fac Ciencias Farmaceut UNESP, Dept Farmacos Medicamentos, BR-14801902 Araraquara, SP, Brazil
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2015年 / 15卷 / 01期
关键词：
Nanostructured Emulsion; Copaiba (Copaifera langsdorffii) Oil; Bullfrog (Rana catesbeiana) Oil; Antimicrobial Activity; COPAIFERA-MULTIJUGA HAYNE; BIOFILM FORMATION; IN-VITRO; TERPENOIDS;
D O I：
10.1166/jnn.2015.9187
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The aim of this work was to investigate the antimicrobial activity of nanostructured emulsions based on copaiba (Copaifera langsdotffii) resin-oil, copaiba essential oil, and bullfrog (Rana catesbeiana Shaw) oil against fungi and bacteria related to skin diseases. Firstly, the essential oil was extracted from copaiba resin-oil and these oils, along with bullfrog oil, were characterized by gas chromatography combined with mass spectrometry (GC-MS). Secondly, nanostructured emulsion systems were produced and characterized. The antimicrobial susceptibility assay was performed, followed by the Minimum Inhibitory Concentration (MIC) determination, the bioautography assay, and the antibiofilm determination. Strains of the genera Staphylococcus, Pseudomonas, and Candida were used. The CG-MS analysis was able to identify the components of copaiba resin-oil, copaiba essential oil, and bullfrog oil. The MIC assay in association with the bioautography revealed that some esters of palmitic and oleic acids, alpha-curcumene, alpha-himachalene, isothujol, and alpha-fenchene-probably inhibited some strains. The nanostructured emulsions based on copaiba resin-oil and essential oil improved the antimicrobial activity of the pure oils, especially against Staphylococcus and Candida, resistant to azoles. The bullfrog oil nanostructured emulsion showed a lower antimicrobial effect when compared to the copaiba samples. However, bullfrog oil-based nanostructured emulsion showed a significant antibiofilm activity (p<0.05). Given the significant antimicrobial and antibiofilm activities of the evaluated oils, it may be concluded that nanostructured emulsions based on copaiba and bullfrog oils are promising candidates for the treatment of infections and also may be used to incorporate other antimicrobial drugs.
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页码：880 / 888
页数：9
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