Two-dimensional biochemical imaging sensor for spatially resolved glutamate monitoring

被引:11
作者
Bang, L
Tan, WH [1 ]
机构
[1] Univ Florida, Dept Chem, Gainesville, FL 32611 USA
[2] Univ Florida, UF Brain Inst, Gainesville, FL 32611 USA
基金
美国国家科学基金会;
关键词
two-dimensional biochemical sensor; glutamate; spatially resolved imaging and sensing;
D O I
10.1016/S0003-2670(99)00523-1
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A two-dimensional biochemical sensor has been developed for spatially resolved imaging and sensing with 0.2 mu M glutamate sensitivity and fast response. The glutamate sensor was made by covalently immobilizing glutamate dehydrogenase onto hat silica plates. The two-dimensional sensor has the advantage of capturing simultaneous measurements at multiple points and may thus allow better understanding of many biological and chemical processes through spatially resolved sensing and imaging. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:91 / 94
页数:4
相关论文
共 11 条
[1]   Chemical analysis of single cells and exocytosis [J].
Chen, GY ;
Ewing, AG .
CRITICAL REVIEWS IN NEUROBIOLOGY, 1997, 11 (01) :59-90
[2]   Direct immobilization of glutamate dehydrogenase on optical fiber probes for ultrasensitive glutamate detection [J].
Cordek, J ;
Wang, XW ;
Tan, WH .
ANALYTICAL CHEMISTRY, 1999, 71 (08) :1529-1533
[3]   DIRECT MEASUREMENT OF GLUTAMATE RELEASE IN THE BRAIN USING A DUAL ENZYME-BASED ELECTROCHEMICAL SENSOR [J].
HU, YB ;
MITCHELL, KM ;
ALBAHADILY, FN ;
MICHAELIS, EK ;
WILSON, GS .
BRAIN RESEARCH, 1994, 659 (1-2) :117-125
[4]   High temporal resolution monitoring of glutamate and aspartate in vivo using microdialysis on-line with capillary electrophoresis with laser-induced fluorescence detection [J].
Lada, MW ;
Vickroy, TW ;
Kennedy, RT .
ANALYTICAL CHEMISTRY, 1997, 69 (22) :4560-4565
[5]   Solution-based analysis of multiple analytes by a sensor array: Toward the development of an "electronic tongue" [J].
Lavigne, JJ ;
Savoy, S ;
Clevenger, MB ;
Ritchie, JE ;
McDoniel, B ;
Yoo, SJ ;
Anslyn, EV ;
McDevitt, JT ;
Shear, JB ;
Neikirk, D .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (25) :6429-6430
[6]   Randomly ordered addressable high-density optical sensor arrays [J].
Michael, KL ;
Taylor, LC ;
Schultz, SL ;
Walt, DR .
ANALYTICAL CHEMISTRY, 1998, 70 (07) :1242-1248
[7]   Monitoring the reactions of single enzyme molecules and single metal ions [J].
Tan, WH ;
Yeung, ES .
ANALYTICAL CHEMISTRY, 1997, 69 (20) :4242-4248
[8]   SUBMICROMETER INTRACELLULAR CHEMICAL OPTICAL FIBER SENSORS [J].
TAN, WH ;
SHI, ZY ;
SMITH, S ;
BIRNBAUM, D ;
KOPELMAN, R .
SCIENCE, 1992, 258 (5083) :778-781
[9]   NEUROTRANSMITTER IMAGING IN LIVING CELLS BASED ON NATIVE FLUORESCENCE DETECTION [J].
TAN, WH ;
PARPURA, V ;
HAYDON, PG ;
YEUNG, ES .
ANALYTICAL CHEMISTRY, 1995, 67 (15) :2575-2579
[10]   Simultaneous detection of catecholamine exocytosis and Ca2+ release from single bovine chromaffin cells using a dual microsensor [J].
Xin, Q ;
Wightman, RM .
ANALYTICAL CHEMISTRY, 1998, 70 (09) :1677-1681