Bifunctional role of a pattern recognition molecule β-1,3 glucan binding protein purified from mangrove crab Episesarma tetragonum

被引：18
作者：
Sivakamavalli, Jeyachandran [1 ]
Vaseeharan, Baskaralingam [1 ]
机构：
[1] Alagappa Univ, Dept Anim Hlth & Management, Crustacean Mol Biol & Genom Lab, Karaikkudi 630004, Tamil Nadu, India
来源：
JOURNAL OF INVERTEBRATE PATHOLOGY | 2014年 / 119卷
关键词：
Agglutination; beta-Glucan binding protein; Episesarma tetragonum; Innate immunity; Prophenoloxidase; PLASMA-PROTEIN; TIGER SHRIMP; PROPHENOLOXIDASE; PURIFICATION; ACTIVATION; HEMOLYMPH; SYSTEM; IDENTIFICATION; SILKWORM; CLONING;
D O I：
10.1016/j.jip.2014.02.013
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
beta-1,3-Glucan binding protein (beta-GBP) was purified from the haemolymph of Episesarma tetragonum by affinity chromatography with epoxy-activated laminarin-sepharose CL-6B column. E. tetragonum beta-GBP exhibits a single band with a molecular weight of 100 kDa on SDS-PAGE and a pI of 5.9 in isoelectric focusing (IEF). The circular dichroism (CD) spectra result of E. tetragonum beta-GBP indicates that the negative ellipticity bands near 220 nm and 208 nm correspond to the beta-sheets in the secondary structure. Functional analysis results demonstrate that the purified E. tetragonum beta-GBP agglutinates fungal cells (Candida albicans) containing p-glucan. This recognition and binding specificity leads to the activation of the prophenoloxidase (ProPO) cascade and enhance the phenoloxidase (PO) activity in a dose-dependent manner. Our finding discloses new insights in the ProPO activation and fungal agglutination of purified E. tetragonum beta-GBP. It seems to play a significant role in microbial uncovering mechanism in invertebrates. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：25 / 31
页数：7
相关论文
共 30 条
[1]   Identification and cloning of a glucan- and liopoplysaccharide-binding protein from Eisenia foetida earthworm involved in the activation of prophenoloxidase cascade [J].
Beschin, A ;
Bilej, M ;
Hanssens, F ;
Raymakers, J ;
Van Dyck, E ;
Revets, H ;
Brys, L ;
Gomez, J ;
De Baetselier, P ;
Timmermans, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (38) :24948-24954
[2]  
CERENIUS L, 1994, J BIOL CHEM, V269, P29462
[3]   Identification, purification and properties of a β-1,3-glucan-specific lectin from the serum of the cockroach, Blaberus discoidalis which is implicated in immune defence reactions [J].
Chen, CL ;
Rowley, AF ;
Newton, RP ;
Ratcliffe, NA .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1999, 122 (03) :309-319
[4]   Comparative and functional genomics of the innate immune system in the malaria vector Anopheles gambiae [J].
Christophides, GK ;
Vlachou, D ;
Kafatos, FC .
IMMUNOLOGICAL REVIEWS, 2004, 198 :127-148
[5]   BETA-1,3-GLUCAN-BINDING PROTEINS FROM PLASMA OF THE FRESH-WATER CRAYFISHES ASTACUS-ASTACUS AND PROCAMBARUS-CLARKII [J].
DUVIC, B ;
SODERHALL, K .
JOURNAL OF CRUSTACEAN BIOLOGY, 1993, 13 (03) :403-408
[6]  
DUVIC B, 1990, J BIOL CHEM, V265, P9327
[7]   Peptidoglycan recognition proteins (PGRPs) [J].
Dziarski, R .
MOLECULAR IMMUNOLOGY, 2004, 40 (12) :877-886
[8]   cDNA cloning, purification, properties, and function of a β-1,3-glucan recognition protein from a pyralid moth, Plodia interpunctella [J].
Fabrick, JA ;
Baker, JE ;
Kanost, MR .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2003, 33 (06) :579-594
[9]   The synthesis of an analogue of the locust CRF-like diuretic peptide, and the biological activities of this and some C-terminal fragments [J].
Goldsworthy, GJ ;
Chung, JS ;
Simmonds, MSJ ;
Tatari, M ;
Varouni, S ;
Poulos, CP .
PEPTIDES, 2003, 24 (10) :1607-1613
[10]   Evidence for a novel biological role for the multifunctional β-1,3-glucan binding protein in shrimp [J].
Goncalves, Priscila ;
Vernal, Javier ;
Rosa, Rafael Diego ;
Yepiz-Plascencia, Gloria ;
Batista de Souza, Claudia Regina ;
Barracco, Margherita Anna ;
Perazzolo, Luciane Maria .
MOLECULAR IMMUNOLOGY, 2012, 51 (3-4) :363-367
123