Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli

被引:19
作者
Ranfaing, Jeremy [1 ]
Dunyach-Remy, Catherine [1 ,2 ]
Louis, Laurence [3 ]
Lavigne, Jean-Philippe [1 ,2 ]
Sotto, Albert [1 ,4 ]
机构
[1] Univ Montpellier, Fac Med, French Natl Inst Hlth & Med Res, Unit 1047, F-30908 Nimes, France
[2] Nimes Univ Hosp, Dept Microbiol, F-30029 Nimes, France
[3] Univ Aix Marseille, Fac Med La Timone, UMR S 910, Plateforme Genom & Transcript, F-13385 Marseille, France
[4] Nimes Univ Hosp, Dept Infect Dis, F-30029 Nimes, France
关键词
URINARY-TRACT-INFECTIONS; BIOFILM FORMATION; ANTIBACTERIAL ACTIVITY; IN-VITRO; EXPRESSION; PROANTHOCYANIDINS; ADHESION; GENES; MOTILITY; ROLES;
D O I
10.1038/s41598-018-29082-6
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Uropathogenic Escherichia coli (UPEC), the most prevalent bacteria isolated in urinary tract infections (UTI), is now frequently resistant to antibiotics used to treat this pathology. The antibacterial properties of cranberry and propolis could reduce the frequency of UTIs and thus the use of antibiotics, helping in the fight against the emergence of antibiotic resistance. Transcriptomic profiles of a clinical UPEC strain exposed to cranberry proanthocyanidins alone (190 mu g/mL), propolis alone (102.4 mu g/mL) and a combination of both were determined. Cranberry alone, but more so cranberry + propolis combined, modified the expression of genes involved in different essential pathways: down-expression of genes involved in adhesion, motility, and biofilm formation, and up-regulation of genes involved in iron metabolism and stress response. Phenotypic assays confirmed the decrease of motility (swarming and swimming) and biofilm formation (early formation and formed biofilm). This study showed for the first time that propolis potentiated the effect of cranberry proanthocyanidins on adhesion, motility, biofilm formation, iron metabolism and stress response of UPEC. Cranberry + propolis treatment could represent an interesting new strategy to prevent recurrent UTI.
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页数:11
相关论文
共 68 条
[1]   Loss of fimbrial adhesion with the addition of Vaccinum macrocarpon to the growth medium of β-fimbriated Escherichia coli [J].
Ahuja, S ;
Kaack, B ;
Roberts, J .
JOURNAL OF UROLOGY, 1998, 159 (02) :559-562
[2]   IN VITRO AND IN VIVO ANALYSIS OF THE ARCB/A REDOX SIGNALING PATHWAY [J].
Alvarez, Adrian F. ;
Georgellis, Dimitris .
METHODS IN ENZYMOLOGY, VOL 471: TWO-COMPONENT SIGNALING SYSTEMS, PART C, 2010, 471 :205-228
[3]   Bacterial iron homeostasis [J].
Andrews, SC ;
Robinson, AK ;
Rodríguez-Quiñones, F .
FEMS MICROBIOLOGY REVIEWS, 2003, 27 (2-3) :215-237
[4]   Genetic Diversity of the fliC Genes Encoding the Flagellar Antigen H19 of Escherichia coli and Application to the Specific Identification of Enterohemorrhagic E-coli O121:H19 [J].
Beutin, Lothar ;
Delannoy, Sabine ;
Fach, Patrick .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2015, 81 (12) :4224-4230
[5]   Antibacterial Activity of a Cardanol from Thai Apis mellifera Propolis [J].
Boonsai, Pattaraporn ;
Phuwapraisirisan, Preecha ;
Chanchao, Chanpen .
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, 2014, 11 (04) :327-336
[6]   Function of proline residues of MotA in torque generation by the flagellar motor of Escherichia coli [J].
Braun, TF ;
Poulson, S ;
Gully, JB ;
Empey, JC ;
van Way, S ;
Putnam, A ;
Blair, DF .
JOURNAL OF BACTERIOLOGY, 1999, 181 (11) :3542-3551
[7]  
Bruyere F, 2017, MED MALADIES INFECT, V47, pS109
[8]  
Buchanan SK, 1999, NAT STRUCT BIOL, V6, P56
[9]   EfeUOB (YcdNOB) is a tripartite, acid-induced and CpxAR-regulated, low-pH Fe2+ transporter that is cryptic in Escherichia coli K-12 but functional in E-coli O157:H7 [J].
Cao, Jieni ;
Woodhall, Mark R. ;
Alvarez, Javier ;
Cartron, Michael L. ;
Andrews, Simon C. .
MOLECULAR MICROBIOLOGY, 2007, 65 (04) :857-875
[10]   Feo - Transport of ferrous iron into bacteria [J].
Cartron, Michael L. ;
Maddocks, Sarah ;
Gillingham, Paul ;
Craven, C. Jeremy ;
Andrews, Simon C. .
BIOMETALS, 2006, 19 (02) :143-157