Platform for Controlled Supramolecular Nanoassembly

被引：14

作者：

Czolkos, Ilja ^{[1
]}

Hannestad, Jonas K. ^{[1
]}

Jesorka, Aldo ^{[1
]}

Kumar, Ravindra ^{[2
]}

Brown, Tom ^{[2
]}

Albinsson, Bo ^{[1
]}

Orwar, Owe ^{[1
]}

机构：

[1] Chalmers, Dept Biol & Chem Engn, S-41296 Gothenburg, Sweden

[2] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

来源：

NANO LETTERS | 2009年 / 9卷 / 06期

基金：

欧洲研究理事会;

关键词：

LIPID-BILAYERS; DNA; FLUORESCENCE; KINETICS; NANOSTRUCTURES; RESOLUTION; DROPLET;

D O I：

10.1021/nl901254f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We here present a two-dimensional (2D) micro/nano-fluidic technique where reactant-doped liquid-crystal films spread and mix on micro- and nanopatterned substrates. Surface-supported phospholipid monolayers are individually doped with complementary DNA molecules which hybridize when these lipid films mix. Using lipid films to convey reactants reduces the dimensionality of traditional 3D chemistry to 2D, and possibly to 1D by confining the lipid film to nanometer-sized lanes. The hybridization event was observed by FRET using single-molecule-sensitive confocal fluorescence detection. We could successfully detect hybridization in lipid streams on 250 nm wide lanes. Our results show that the number and density of reactants as well as sequence of reactant addition can be controlled within confined liquid crystal films, providing a platform for nanochemistry with potential for kinetic control.

引用

页码：2482 / 2486

页数：5

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