Colorimetric detection of human chorionic gonadotropin using catalytic gold nanoparticles and a peptide aptamer

被引:54
作者
Chang, Chia-Chen [1 ]
Chen, Chie-Pein [2 ]
Lee, Chung-Han [1 ]
Chen, Chen-Yu [2 ,3 ,4 ]
Lin, Chii-Wann [1 ,5 ,6 ]
机构
[1] Natl Taiwan Univ, Inst Biomed Engn, Taipei 106, Taiwan
[2] Mackay Mem Hosp, Dept Obstet & Gynecol, Taipei 104, Taiwan
[3] Mackay Jr Coll Med Nursing & Management, Taipei 112, Taiwan
[4] Mackay Med Coll, Taipei 252, Taiwan
[5] Natl Taiwan Univ, Inst Biomed Elect & Bioinformat, Taipei 106, Taiwan
[6] Natl Taiwan Univ, Ctr Emerging Mat & Adv Devices, Taipei 106, Taiwan
关键词
GERM-CELL TUMORS; AMPEROMETRIC IMMUNOSENSOR; PROTEIN BIOMARKERS; ECTOPIC PREGNANCY; HCG; FLUIDS; ASSAY; EXPERIENCE; DIAGNOSIS; GRAPHENE;
D O I
10.1039/c4cc06366j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We combined catalytic gold nanoparticles (AuNPs) with an hCG-specific peptide aptamer to create a simple, sensitive, label-free colorimetric assay for hCG. The applications of this colorimetric biosensor may be expanded by changing the peptide aptamer.
引用
收藏
页码:14443 / 14446
页数:4
相关论文
共 42 条
[1]   Surface chemistry to minimize fouling from blood-based fluids [J].
Blaszykowski, Christophe ;
Sheikh, Sonia ;
Thompson, Michael .
CHEMICAL SOCIETY REVIEWS, 2012, 41 (17) :5599-5612
[2]   DIAGNOSIS OF ECTOPIC PREGNANCY BY VAGINAL ULTRASONOGRAPHY IN COMBINATION WITH A DISCRIMINATORY SERUM HCG LEVEL OF 1000-IU/1 (IRP) [J].
CACCIATORE, B ;
STENMAN, UH ;
YLOSTALO, P .
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 1990, 97 (10) :904-908
[3]   Case report - Testicular Leydig's cell hyperplasia: MR imaging and sonographic findings [J].
Carucci, LR ;
Tirkes, AT ;
Pretorius, ES ;
Genega, EM ;
Weinstein, SP .
AMERICAN JOURNAL OF ROENTGENOLOGY, 2003, 180 (02) :501-503
[4]   Label-free colorimetric aptasensor for IgE using DNA pseudoknot probe [J].
Chang, Chia-Chen ;
Chen, Chen-Yu ;
Zhao, Xihong ;
Wu, Tzu-Heng ;
Wei, Shih-Chung ;
Lin, Chii-Wann .
ANALYST, 2014, 139 (13) :3347-3351
[5]   Aptamer-based colorimetric detection of platelet-derived growth factor using unmodified gold nanoparticles [J].
Chang, Chia-Chen ;
Wei, Shih-Chung ;
Wu, Tzu-Heng ;
Lee, Chung-Han ;
Lin, Chii-Wann .
BIOSENSORS & BIOELECTRONICS, 2013, 42 :119-123
[6]   Amplified surface plasmon resonance immunosensor for interferon-Gamma based on a streptavidin-incorporated aptamer [J].
Chang, Chia-Chen ;
Lin, Shenhsiung ;
Lee, Chung-Han ;
Chuang, Tsung-Liang ;
Hsueh, Po-Ren ;
Lai, Hsin-Chih ;
Lin, Chii-Wann .
BIOSENSORS & BIOELECTRONICS, 2012, 37 (01) :68-74
[7]   An amplified surface plasmon resonance "turn-on" sensor for mercury ion using gold nanoparticles [J].
Chang, Chia-Chen ;
Lin, Shenhsiung ;
Wei, Shih-Chung ;
Chen, Chen-Yu ;
Lin, Chii-Wann .
BIOSENSORS & BIOELECTRONICS, 2011, 30 (01) :235-240
[8]   PREGNANCY TESTS - A REVIEW [J].
CHARD, T .
HUMAN REPRODUCTION, 1992, 7 (05) :701-710
[9]   Applications of Aptamers as Sensors [J].
Cho, Eun Jeong ;
Lee, Joo-Woon ;
Ellington, Andrew D. .
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, 2009, 2 :241-264
[10]   Background hCG in non-pregnant individuals: Need for more sensitive point-of-care and over-the-counter pregnancy tests [J].
Cole, Laurence A. ;
Ladner, Donald G. .
CLINICAL BIOCHEMISTRY, 2009, 42 (03) :168-175