Nanocellulose Films to Improve the Performance of Distance-based Glucose Detection in Paper-based Microfluidic Devices

被引：19

作者：

Prapaporn, Sangkaew ^{[1
]}

Arisara, Sriruangrungkamol ^{[2
]}

Wunpen, Chonkaew ^{[2
]}

Wijitar, Dungchai ^{[1
]}

机构：

[1] King Mongkuts Univ Technol Thonburi, Fac Sci, Dept Chem, Organ Synth Electrochem & Nat Prod Res Unit, Prachautid Rd, Bangkok 10140, Thailand

[2] King Mongkuts Univ Technol Thonburi, Fac Sci, Dept Chem, Prachautid Rd, Bangkok 10140, Thailand

来源：

ANALYTICAL SCIENCES | 2020年 / 36卷 / 12期

关键词：

Distance-based paper device (dPAD); glucose; nanocellulose; silver nanoparticles (AgNPs); SENSITIVE COLORIMETRIC DETECTION; HYDROGEN-PEROXIDE; URIC-ACID; PLATFORM; NANOPARTICLES; BLOOD; ASSAY; BIO; AG;

D O I：

10.2116/analsci.20P168

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report on a simple, cost-effective, instrument-free, and portable distance-based paper device coupled with NFs for the determination of glucose. The analysis reaction is based upon the oxidative etching reaction of silver nanoparticles (AgNPs) in the presence of H2O2 that is produced from glucose after a glucose oxidase (GOx) catalytic reaction leading to a morphological transformation of AgNPs. A color band length of AgNPs is coated on to a detection channel and then etched by H2O2, and these were changed from a purple color to colorless as a correlate of the glucose concentration. To improve the performance of the enzyme immobilization, NFs, which are biocompatible without compromising their structure and biological activity, were then placed onto the sample zone. The naked-eye detection limit was 0.1 mM for 40 mM of analysis time. The recoveries of glucose spiked in the artificial urine samples and control urine samples were then verified by our device and were in the acceptable range of 96 - 100%.

引用

页码：1447 / 1452

页数：6

共 39 条

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