PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiania, GO

被引：17

作者：

Carvalho, Rosangela Nunes ^{[1
]}

de Oliveira, Antonio Nonato ^{[2
]}

de Mesquita, Albenones Jose ^{[2
]}

Minafra e Rezende, Cintia Silva ^{[2
]}

de Mesquita, Adriano Queiroz ^{[2
]}

Mazzoni Romero, Rolando Alfredo ^{[2
]}

机构：

[1] Embrapa Arroz & Feijao, Santo Antonio De Goias, Go, Brazil

[2] Univ Fed Goias, Ctr Pesquisa Alimentos, Escola Vet & Zootecnia, BR-74690900 Goiania, Go, Brazil

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2014年 / 45卷 / 01期

关键词：

Minas Frescal cheese; STEC/VTEC; PCR; VIDAS ECO O157 (R); RAW-MILK; DAIRY-CATTLE; SAO-PAULO; STEC; O157-H7; PREVALENCE; SEROTYPES; GENES; STATE;

D O I：

10.1590/S1517-83822014000100002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157 (R), bioM rieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiania and thirty from industries under Federal Inspection located in Goias State which trade their products in supermarkets in Goiania. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection.

引用

页码：7 / 10

页数：4

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