Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics

被引：7

作者：

Schulte-Zweckel, Janine ^{[1
]}

Rosi, Federica ^{[1
]}

Sreenu, Domalapally ^{[1
]}

Schroeder, Hendrik ^{[2
]}

Niemeyer, Christof M. ^{[2
,3
]}

Triola, Gemma ^{[1
,4
]}

机构：

[1] Max Planck Inst Mol Physiol, Abt Chem Biol, D-44227 Dortmund, Germany

[2] Tech Univ Dortmund, Fak Chem Biol Chem Mikrostrukturtech, D-44227 Dortmund, Germany

[3] Karlsruhe Inst Technol, D-76344 Eggenstein Leopoldshafen, Germany

[4] Inst Adv Chem Catalonia, Dept Biomed Chem, Barcelona 08034, Spain

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 84期

关键词：

TETRACYSTEINE-TAGGED PROTEINS; CELLS; CHEMISTRY; FLASH;

D O I：

10.1039/c4cc04120h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without the need for secondary detection methods.

引用

页码：12761 / 12764

页数：4

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