Four new eremophilane-type sesquiterpenes, 3 beta-(acetyloxy)-6 beta-(2-methylbutanoyloxy)-10 beta-hydroxy-eremophil-7(11)-en-12,8 alpha-olide (1), 3 beta-(acetyloxy)-6 alpha,7 alpha-epoxy-11-noreremophil-9(10)-ene-8,11-dione (2), 3 beta-hydroxy-11-noreremophila-6(7),9(10)-diene-8,11-dione (3), and 3 beta-(3-methylbutanoyloxy)-11-noroxoeremophila-6(7),9(10)-diene-8,11-dione (4) were isolated from roots of Ligularia japonica. The structures of the new compounds were elucidated through spectral studies including HR-EI-MS, IR, and NMR data.