Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast

被引：37

作者：

Aihara, Masamune ^{[1
]}

Jin, Xiulian ^{[2
]}

Kurihara, Yusuke ^{[1
,2
]}

Yoshida, Yutaka ^{[3
]}

Matsushima, Yuichi ^{[1
]}

Oku, Masahide

Hirota, Yuko ^{[2
]}

Saigusa, Tetsu ^{[2
]}

Aoki, Yoshimasa ^{[1
]}

Uchiumi, Takeshi ^{[1
]}

Yamamoto, Tadashi ^{[3
]}

Sakai, Yasuyoshi ^{[4
]}

Kang, Dongchon ^{[1
]}

Kanki, Tomotake ^{[2
]}

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Dept Clin Chem & Lab Med, Fukuoka 8128582, Japan

[2] Niigata Univ, Grad Sch Med & Dent Sci, Lab Biosignaling, Niigata 9518510, Japan

[3] Niigata Univ, Grad Sch Med & Dent Sci, Inst Nephrol, Dept Struct Pathol, Niigata 9518510, Japan

[4] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Kyoto 6068502, Japan

来源：

JOURNAL OF CELL SCIENCE | 2014年 / 127卷 / 14期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Autophagy; Mitophagy; Mitochondria; Yeast; Atg32; Tor; Sin3; Rpd3; MITOCHONDRIAL PROTEIN; AUTOPHAGY; PHOSPHORYLATION; DEGRADATION; HOMOLOG; UME6;

D O I：

10.1242/jcs.153254

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

When mitophagy is induced in Saccharomyces cerevisiae, the mitochondrial outer membrane protein ScAtg32 interacts with the cytosolic adaptor protein ScAtg11. ScAtg11 then delivers the mitochondria to the pre-autophagosomal structure for autophagic degradation. Despite the importance of ScAtg32 for mitophagy, the expression and functional regulation of ScAtg32 are poorly understood. In this study, we identified and characterized the ScAtg32 homolog in Pichia pastoris (PpAtg32). Interestingly, we found that PpAtg32 was barely expressed before induction of mitophagy and was rapidly expressed after induction of mitophagy by starvation. Additionally, PpAtg32 was phosphorylated when mitophagy was induced. We found that PpAtg32 expression was suppressed by Tor and the downstream PpSin3-PpRpd3 complex. Inhibition of Tor by rapamycin induced PpAtg32 expression, but could neither phosphorylate PpAtg32 nor induce mitophagy. Based on these findings, we conclude that the Tor and PpSin3-PpRpd3 pathway regulates PpAtg32 expression, but not PpAtg32 phosphorylation.

引用

页码：3184 / 3196

页数：13

共 29 条

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