The protective effect of clay minerals against damage to adsorbed DNA induced by cadmium and mercury

被引：33

作者：

Hou, Yakun ^{[1
]}

Wu, Pingxiao ^{[1
,2
,3
]}

Zhu, Nengwu ^{[1
,2
,3
]}

机构：

[1] S China Univ Technol, Coll Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China

[2] Minist Educ, Key Lab Pollut Control & Ecosyst Restorat Ind Clu, Guangzhou 510006, Guangdong, Peoples R China

[3] Guangdong Regular Higher Educ Inst, Key Lab Environm Protect & Ecoremediat, Guangzhou 510006, Guangdong, Peoples R China

来源：

CHEMOSPHERE | 2014年 / 95卷

基金：

高等学校博士学科点专项科研基金; 美国国家科学基金会;

关键词：

DNA adsorption; Clay; Adsorption mechanism; Heavy metal; Protection; BIOLOGICAL-ACTIVITY; BACTERIAL-DNA; ADSORPTION; MONTMORILLONITE; BINDING; SOIL; TRANSFORMATION; DEGRADATION; PERSISTENCE; COMPLEXES;

D O I：

10.1016/j.chemosphere.2013.08.069

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The adsorption of Salmon Sperm DNA on three kinds of raw clay (rectorite, montmorillonite and sericite) was investigated as a function of pH, ionic strength and the concentrations of DNA and phosphate ions in solution. The DNA adsorption was reduced in the following order: rectorite > montmorillonite > sericite. Based on these findings, there is a strong evidence that the mechanisms for DNA adsorption on clay involve electrostatic forces, cation bridging and ligand exchange. Cyclic voltammetry (CV) and UV-vis absorption and fluorescence spectroscopy were used to compare the properties of unbound DNA and the absorbed DNA on rectorite, both in the absence and presence of Cd2+ and Hg2+ inaqueous solutions. The interaction of heavy metals with the unbound DNA was evidenced by the disappearance of reduction peaks in CV, a small bathochromic shift in UV-vis spectroscopy and an incomplete quenching in the emission spectra. Such changes were not observed in the DNA-rectorite hybrids, which is evidence that adsorption on the clay can reduce the extent of the DNA damage caused by heavy metals. Therefore, in these experience the rectorite played an important role in protecting DNA against Cd2+ and Hg2+ induced damage. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：206 / 212

页数：7

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