Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers

被引:35
|
作者
Nateche, F [1 ]
Martin, A
Baraka, S
Palomino, JC
Khaled, S
Portaels, F
机构
[1] Univ Sci & Technol, Fac Biol, Algiers, Algeria
[2] Lab Cent Esh Hadi Flici, Algiers, Algeria
[3] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium
关键词
D O I
10.1099/jmm.0.46513-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study assessed the performance of a rapid, low-cost, colorimetric method, the resazurin microtitre assay (REMA) plate method, for the detection of resistance to isoniazid and rifampicin in 136 clinical isolates of Mycobacterium tuberculosis from two hospitals in Algiers. MICs were determined and the results were compared with those obtained with the conventional proportion method on Lowenstein-Jensen medium. Excellent results were obtained for the REMA plate method, with a sensitivity of 100% for both isoniazid and rifampicin and a specificity of 98(.)3 and 99(.)2 %, respectively. The REMA plate method appears to be a reliable method for the rapid determination of multidrug-resistant tuberculosis and is a good alternative for use in resource-limited countries such as Algeria.
引用
收藏
页码:857 / 860
页数:4
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