Novel metrics to classify fire blight resistance of 94 apple cultivars

被引:34
作者
Kostick, S. A. [1 ]
Norelli, J. L. [2 ]
Evans, K. M. [1 ]
机构
[1] Washington State Univ, Dept Hort, Tree Fruit Res & Extens Ctr, Wenatchee, WA 98801 USA
[2] ARS, USDA, Appalachian Fruit Res Lab, Kearneysville, WV 25430 USA
关键词
Erwinia amylovora; Malus x domestica; Malus pumila; shoot length blighted (SLB); QUANTITATIVE TRAIT LOCI; ERWINIA-AMYLOVORA; MAJOR QTL; BACTERIAL ANTAGONISTS; IDENTIFICATION; ESTABLISHMENT; VIRULENCE; BLOSSOMS; STRAINS; PEAR;
D O I
10.1111/ppa.13012
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Fire blight (Erwinia amylovora), a potentially devastating disease in apple, can cause floral, fruit and structural damage and even tree death. Most commercial apple cultivars are susceptible and the resistance/susceptibility of many modern cultivars has not been evaluated. Fire blight resistance/susceptibility is difficult to phenotype due to quantitative resistance, impacts of tree vigour and environment on susceptibility, and the erratic nature of the disease. Resistance/susceptibility levels were determined for 94 apple cultivars and important breeding parents. In 2016 and 2017, multiple actively growing shoots per tree (about three trees per cultivar) were challenged with E. amylovora Ea153n via a cut-leaf inoculation method. Proportion of current season's shoot length blighted (SLB) was calculated for each shoot. To classify cultivar responses, estimated marginal SLB means were compared to four controls, representing highly susceptible (HS) to highly resistant (HR), via Dunnett's tests. Cultivar responses ranged from HS to HR with estimated marginal SLB means of 0.001-0.995 in 2016 and 0.000-0.885 in 2017. Most cultivars demonstrated similar resistance/susceptibility levels in both years (rho = 0.657, P 0.0001). K-means clustering was used to classify cultivars into three resistance/susceptibility groups based on incidence, average severity (SLB), and maximum severity values (maximum SLB and age of wood infected). Sixteen cultivars were consistently moderately resistant (MR) to HR while the remainder ranged from HS to MR. An updated comparison of susceptibility of important cultivars is provided. Resistance/susceptibility information gained could be used to identify genetic loci associated with resistance/susceptibility and/or inform parental selection in apple scion breeding programmes.
引用
收藏
页码:985 / 996
页数:12
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