Power-Law Stress and Creep Relaxations of Single Cells Measured by Colloidal Probe Atomic Force Microscopy

被引：30

作者：

Hiratsuka, Shinichiro ^{[1
]}

Mizutani, Yusuke ^{[1
]}

Toda, Akitoshi ^{[2
]}

Fukushima, Norichika ^{[2
]}

Kawahara, Koichi ^{[1
]}

Tokumoto, Hiroshi ^{[3
]}

Okajima, Takaharu ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Informat Sci & Technol, Sapporo, Hokkaido 0600814, Japan

[2] Olympus Corp, MEMS Technol Div, Tokyo 1928512, Japan

[3] Hokkaido Univ, Res Inst Elect Sci, Nanotechnol Res Ctr, Sapporo, Hokkaido 0010021, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2009年 / 48卷 / 08期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

VISCOELASTIC PROPERTIES; RHEOLOGICAL BEHAVIOR; LIVING CELLS; LUNG; ELASTICITY; MECHANICS;

D O I：

10.1143/JJAP.48.08JB17

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We measured stress and creep relaxations of mouse fibroblast cells arranged and cultured on a microarray, by colloidal probe atomic force microscopy (AFM). A hydrophobic monolayer coating of perfluorodecyltrichlorosilane (FDTS) on the surface of colloidal silica beads significantly reduced the adhesion force of live cells, compared with untreated beads. The rheological behaviors of cells were estimated by averaging several relaxation curves of cells measured by the AFM. Longer-time tailing of both stress and creep relaxation curves followed single power-law behavior over a time scale of 60s, with exponents in the range 0.1-0.4, varying with cells. The results were in good agreement with previous measurements of the frequency-domain rheology of cells using the force modulation mode. (C) 2009 The Japan Society of Applied Physics

引用

页数：4

共 33 条

[1] Microrheology of human lung epithelial cells measured by atomic force microscopy [J].

Alcaraz, J ;

Buscemi, L ;

Grabulosa, M ;

Trepat, X ;

Fabry, B ;

Farré, R ;

Navajas, D .

BIOPHYSICAL JOURNAL, 2003, 84 (03) :2071-2079

[2]

[Anonymous], 2009, Theory of Elasticity

[3]

[Anonymous], CREEP RELAXATION NON

[4] Power laws in microrheology experiments on living cells:: Comparative analysis and modeling [J].

Balland, Martial ;

Desprat, Nicolas ;

Icard, Delphine ;

Fereol, Sophie ;

Asnacios, Atef ;

Browaeys, Julien ;

Henon, Sylvie ;

Gallet, Francois .

PHYSICAL REVIEW E, 2006, 74 (02)

[5] Viscoelastic properties of zonal articular chondrocytes measured by atomic force microscopy [J].

Darling, E. M. ;

Zauscher, S. ;

Guilak, F. .

OSTEOARTHRITIS AND CARTILAGE, 2006, 14 (06) :571-579

[6] A thin-layer model for viscoelastic, stress-relaxation testing of cells using atomic force microscopy: Do cell properties reflect metastatic potential? [J].

Darling, Eric M. ;

Zauscher, Stefan ;

Block, Joel A. ;

Guilak, Farshid .

BIOPHYSICAL JOURNAL, 2007, 92 (05) :1784-1791

[7] Fast and slow dynamics of the cytoskeleton [J].

Deng, Linhong ;

Trepat, Xavier ;

Butler, James P. ;

Millet, Emil ;

Morgan, Kathleen G. ;

Weitz, David A. ;

Fredberg, Jeffrey J. .

NATURE MATERIALS, 2006, 5 (08) :636-640

[8] Creep function of a single living cell [J].

Desprat, N ;

Richert, A ;

Simeon, J ;

Asnacios, A .

BIOPHYSICAL JOURNAL, 2005, 88 (03) :2224-2233

[9] Hydrophobic coatings for MEMS applications [J].

Doms, M. ;

Feindt, H. ;

Kuipers, W. J. ;

Shewtanasoontorn, D. ;

Matar, A. S. ;

Brinkhues, S. ;

Welton, R. H. ;

Mueller, J. .

JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2008, 18 (05)

[10] Time scale and other invariants of integrative mechanical behavior in living cells [J].

Fabry, B ;

Maksym, GN ;

Butler, JP ;

Glogauer, M ;

Navajas, D ;

Taback, NA ;

Millet, EJ ;

Fredberg, JJ .

PHYSICAL REVIEW E, 2003, 68 (04)

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