Increased expression of long non-coding RNA PVT1 correlates with clinical progression and poor prognosis in bladder cancer

被引：0

作者：

Cui, Yu ^{[1
]}

Liu, Longfei ^{[1
]}

Chen, Jinbo ^{[1
]}

Li, Chao ^{[1
]}

Cheng, Xu ^{[1
]}

Zu, Xiongbing ^{[1
]}

机构：

[1] Cent South Univ, Xiangya Hosp, Dept Urol, Changsha 410000, Hunan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2017年 / 10卷 / 03期

基金：

中国国家自然科学基金;

关键词：

LncRNA; PVT1; bladder cancer; biomarker; PROMOTES; IDENTIFICATION; AMPLIFICATION; LANDSCAPE; GENOME; TUMOR; CELL;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Several studies have showed that lncRNA PVT1 contributes to cancer progression. However, the relationship between PVT1 and bladder cancer remains uncertain. The aim of this study was to investigate the expression of PVT1 in bladder cancer tissues and further explore its clinical significance. The expression levels of PVT1 were determined using RT-qPCR in a total of 146 patients with bladder cancer. Furthermore, the expression levels of PVT1 were determined in 4 bladder cancer cell lines (RT4, RT112, 5637 and T24) and one normal human uroepithelial cell line (CRL-9520). The association between PVT1 expression and overall survival of bladder cancer patients was evaluated by Kaplan-Meier analysis and log-rank tests. To identify the risk factors associated with overall survival of bladder cancer patients, several factors were evaluated by Cox univariate and multivariate analyses. The results from RT-PCR showed that the expression of PVT1 was not only increased in bladder cancer tissues relative to that in paired adjacent non-tumor tissues, but also upregulated in four bladder cancer cell lines relative to that in a normal human uroepithelial cell line. High PVT1 expression was associated with advanced histological grade, higher tumor stage and positive lymph-node metastasis. Kaplan-Meier survival analysis indicated that high PVT1 expression levels in bladder cancer are significantly associated with worse overall survival. Multivariate analysis showed that high PVT1 expression was an independent prognostic indicator for overall survival of bladder cancer patients. Our study indicated that PVT1 may be considered as a novel prognostic marker and therapeutic target of bladder cancer.

引用

页码：3265 / 3271

页数：7

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