Identification of new transposable genetic elements in Burkholderia pseudomallei using subtractive hybridisation

被引：10
作者：
Brown, NF
Lew, AE
Beacham, IR
机构：
[1] Griffith Univ, Sch Hlth Sci, Gold Coast Mail Ctr, Gold Coast, Qld 4217, Australia
[2] Queensland Dept Primary Ind, Anim Res Inst, Yeerongpilly, Qld 4105, Australia
来源：
FEMS MICROBIOLOGY LETTERS | 2000年 / 183卷 / 01期
关键词：
melioidosis; transposable element; insertion sequence; subtractive hybridisation; fingerprinting; Burkholderia pseudomallei;
D O I：
10.1111/j.1574-6968.2000.tb08936.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A subtraction library of Burkholderia pseudomallei was constructed by subtractive hybridisation of B. pseudomallei genomic DNA with Burkholderia thailandensis genomic DNA. Two clones were found to have significant sequence similarity to insertion sequences which have previously not been found in B. pseudomallei (designated ISA and ISB), and two clones showed sequence similarity to different regions of Burkholderia cepacia IS407 that has recently been detected in B. pseudomallei. The former, though possibly non-functional, represents new transposable genetic elements of B. pseudomallei. All three sequences were found to be present in multi-copy in the genomes of a number of B. pseudomallei strains and in B. thailandensis, which are the first transposable elements identified in this species. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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收藏
页码：73 / 79
页数：7
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